Enzyme-linked immunosorbent assay (ELISA) systems have recently been accepted as validated methods for environmental monitoring. An immunoassay for the detection of tetraconazole, penconazole and their metabolytic derivative (DTP) and a similar assay for the triazole fungicide, myclobutanil have been developed. The present paper describes a comparative study of the two ELISA systems, in regards of sensitivities and cross-reactivities, taking into account two different strategies for the synthesis of the two immunogens. The two sera have been utilized in comparable immobilized antigen formats, and the resultant IC50 values were found to be 1.3 ± 0.4 ppm for tetraconazole in one assay, and 4.9 ± 0.5 ppm for myclobutanil in the other. The antibodies against tetraconazole have also displayed higher selectivity than those raised against the myclobutanil conjugate. Modulation of the assay sensitivity by the level of hapten loading in the antigen coating was also investigated.
|Number of pages||9|
|Journal||Acta Phytopathologica et Entomologica Hungarica|
|Publication status||Published - Dec 1 1996|
ASJC Scopus subject areas
- Plant Science
- Insect Science