Comparative studies on [Ca2+]i-level of fibroblasts from alzheimer patients and control individuals

András Palotás, János Kálmán, Gábor Laskay, Anna Juhász, Zoltán Janka, Botond Penke

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The accumulation of the β-amyloid peptide (βAP) in the brain, produced from the ubiquitously expressed amyloid precursor protein (APP) is a defining feature of Alzheimer's disease (AD). Consistent with studies demonstrating the importance of skin biopsy in the diagnosis of neurodegenerative disorders, we investigated whether differences in intracellular free calcium levels ([Ca2+]i) of cultured cutaneous fibroblasts derived from sporadic AD patients and from age-matched control individuals might be present. [Ca2+]i was measured in Fura-2AM-loaded human fibroblasts by dual wavelength spectrofluorimetry. AD cells exhibited lower [Ca2+]i as compared to the control cultures. Exposure of fibroblasts to βAP resulted in increased [Ca2+]i of the control cells, but not of AD fibroblasts. Our test could prove useful in supporting the diagnosis of (sporadic) AD in patients suspected of suffering from the disease.

Original languageEnglish
Pages (from-to)817-820
Number of pages4
JournalNeurochemical research
Volume26
Issue number7
DOIs
Publication statusPublished - Sep 26 2001

Keywords

  • Alzheimer's disease
  • Fibroblast
  • Fluorescence
  • β-amyloid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Comparative studies on [Ca<sup>2+</sup>]<sub>i</sub>-level of fibroblasts from alzheimer patients and control individuals'. Together they form a unique fingerprint.

  • Cite this