Comparative studies of fresh and postmortem isolated rat cortical synaptosomes: Morphology, K+ transport, and respiration

M. Kálmán, F. Hajos

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4 Citations (Scopus)

Abstract

Synaptosomal fractions were isolated from rat cerebral cortex immediately and at 1, 2, 3, and 4 h postmortem. The capability to undergo function-dependent configurational changes in relation to ouabain- and protoveratrine-sensitive K+ uptake, and the rate of O2 consumption were compared between fresh and postmortem synaptosomal fractions. Our results suggest that a 2 h postmortem storage at room temperature does not decrease substantially the functional performance of synaptosomes, while longer storage periods interfere with their capacity to maintain intracellular cation levels. The findings appear to be relevant to the use of human autopsy material for the preparation of synaptosomes.

Original languageEnglish
Pages (from-to)1740-1742
Number of pages3
JournalJournal of Neurochemistry
Volume39
Issue number6
Publication statusPublished - 1982

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Protoveratrines
Synaptosomes
Ouabain
Cations
Rats
Respiration
Cerebral Cortex
Autopsy
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Comparative studies of fresh and postmortem isolated rat cortical synaptosomes : Morphology, K+ transport, and respiration. / Kálmán, M.; Hajos, F.

In: Journal of Neurochemistry, Vol. 39, No. 6, 1982, p. 1740-1742.

Research output: Contribution to journalArticle

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