Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing

on behalf of the BRaDP1T consortium

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalVeterinary Microbiology
Volume220
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

Actinobacillus pleuropneumoniae
Multiplex Polymerase Chain Reaction
Capsules
Polysaccharides
Sequence Analysis
serotypes
Swine
polysaccharides
sequence analysis
Pleuropneumonia
Molecular Typing
loci
swine
cross reaction
pathogens
assays
Genes
genes
Serogroup
pleuropneumonia

Keywords

  • A. pleuropneumoniae
  • Capsule typing
  • Diagnostic
  • mPCR
  • Serovars

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

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title = "Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing",
abstract = "Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.",
keywords = "A. pleuropneumoniae, Capsule typing, Diagnostic, mPCR, Serovars",
author = "{on behalf of the BRaDP1T consortium} and Boss{\'e}, {Janine T.} and Yanwen Li and {Fernandez Crespo}, Roberto and Sonia Lacouture and Marcelo Gottschalk and Rita S{\'a}rk{\"o}zi and L. Fodor and {Casas Amoribieta}, Maria and {\O}ystein Angen and Katerina Nedbalcova and Holden, {Matthew T.G.} and Maskell, {Duncan J.} and Tucker, {Alexander W.} and Wren, {Brendan W.} and Rycroft, {Andrew N.} and Langford, {Paul R.}",
year = "2018",
month = "7",
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doi = "10.1016/j.vetmic.2018.05.011",
language = "English",
volume = "220",
pages = "83--89",
journal = "Veterinary Microbiology",
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TY - JOUR

T1 - Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing

AU - on behalf of the BRaDP1T consortium

AU - Bossé, Janine T.

AU - Li, Yanwen

AU - Fernandez Crespo, Roberto

AU - Lacouture, Sonia

AU - Gottschalk, Marcelo

AU - Sárközi, Rita

AU - Fodor, L.

AU - Casas Amoribieta, Maria

AU - Angen, Øystein

AU - Nedbalcova, Katerina

AU - Holden, Matthew T.G.

AU - Maskell, Duncan J.

AU - Tucker, Alexander W.

AU - Wren, Brendan W.

AU - Rycroft, Andrew N.

AU - Langford, Paul R.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

AB - Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

KW - A. pleuropneumoniae

KW - Capsule typing

KW - Diagnostic

KW - mPCR

KW - Serovars

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DO - 10.1016/j.vetmic.2018.05.011

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VL - 220

SP - 83

EP - 89

JO - Veterinary Microbiology

JF - Veterinary Microbiology

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