Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays

E. Huszár, Z. Szabó, Á Jakab, I. Barta, I. Herjavecz, I. Horváth

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: Differences between detection techniques may be partly responsible for variable mediator concentrations reported in exhaled breath condensate (EBC). We compared two types of immunoassays to estimate thromboxane A2 (TxA2) concentration. Materials and methods: Thromboxane B2 (TxB2) levels were measured by enzyme immunoassay (EIA) and TxB2/2,3-dinor TxB2 by radioimmunoassay (RIA) in 10 healthy subjects and 13 asthmatic patients. 2,3-Dinor TxB2 was also determined by a separate EIA. Results: Thromboxane was detected in all samples by RIA, but only in about 75 % of samples by EIA. 2,3-Dinor TxB2 was detected in most samples. There was no agreement between the results of the different immunoassays. As compared to healthy subjects, exhaled breath condensates of asthmatic patients contained significantly more immunoreactivity by RIA and TxB2 EIA (but not by 2,3-dinor TxB2 EIA). Conclusion: RIA and EIA resulted in vastly different absolute values. The difference found between healthy volunteers and asthmatic patients however, suggests an increased level of TxA2 in the airways of asthmatics.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalInflammation Research
Volume54
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Thromboxane A2
Metabolites
Immunoenzyme Techniques
Immunoassay
Thromboxane B2
Radioimmunoassay
Enzymes
Healthy Volunteers
Thromboxanes
2,3-dinor-thromboxane B2

Keywords

  • Asthma
  • Enzyme immunoassay
  • Exhaled breath condensate
  • Radioimmunoassay
  • Thromboxane A/B

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays. / Huszár, E.; Szabó, Z.; Jakab, Á; Barta, I.; Herjavecz, I.; Horváth, I.

In: Inflammation Research, Vol. 54, No. 8, 08.2005, p. 350-355.

Research output: Contribution to journalArticle

@article{71d6a9cf797b4e37bca2e80bb8f7be60,
title = "Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays",
abstract = "Objective: Differences between detection techniques may be partly responsible for variable mediator concentrations reported in exhaled breath condensate (EBC). We compared two types of immunoassays to estimate thromboxane A2 (TxA2) concentration. Materials and methods: Thromboxane B2 (TxB2) levels were measured by enzyme immunoassay (EIA) and TxB2/2,3-dinor TxB2 by radioimmunoassay (RIA) in 10 healthy subjects and 13 asthmatic patients. 2,3-Dinor TxB2 was also determined by a separate EIA. Results: Thromboxane was detected in all samples by RIA, but only in about 75 {\%} of samples by EIA. 2,3-Dinor TxB2 was detected in most samples. There was no agreement between the results of the different immunoassays. As compared to healthy subjects, exhaled breath condensates of asthmatic patients contained significantly more immunoreactivity by RIA and TxB2 EIA (but not by 2,3-dinor TxB2 EIA). Conclusion: RIA and EIA resulted in vastly different absolute values. The difference found between healthy volunteers and asthmatic patients however, suggests an increased level of TxA2 in the airways of asthmatics.",
keywords = "Asthma, Enzyme immunoassay, Exhaled breath condensate, Radioimmunoassay, Thromboxane A/B",
author = "E. Husz{\'a}r and Z. Szab{\'o} and {\'A} Jakab and I. Barta and I. Herjavecz and I. Horv{\'a}th",
year = "2005",
month = "8",
doi = "10.1007/s00011-005-1361-x",
language = "English",
volume = "54",
pages = "350--355",
journal = "Inflammation Research",
issn = "1023-3830",
publisher = "Birkhauser Verlag Basel",
number = "8",

}

TY - JOUR

T1 - Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays

AU - Huszár, E.

AU - Szabó, Z.

AU - Jakab, Á

AU - Barta, I.

AU - Herjavecz, I.

AU - Horváth, I.

PY - 2005/8

Y1 - 2005/8

N2 - Objective: Differences between detection techniques may be partly responsible for variable mediator concentrations reported in exhaled breath condensate (EBC). We compared two types of immunoassays to estimate thromboxane A2 (TxA2) concentration. Materials and methods: Thromboxane B2 (TxB2) levels were measured by enzyme immunoassay (EIA) and TxB2/2,3-dinor TxB2 by radioimmunoassay (RIA) in 10 healthy subjects and 13 asthmatic patients. 2,3-Dinor TxB2 was also determined by a separate EIA. Results: Thromboxane was detected in all samples by RIA, but only in about 75 % of samples by EIA. 2,3-Dinor TxB2 was detected in most samples. There was no agreement between the results of the different immunoassays. As compared to healthy subjects, exhaled breath condensates of asthmatic patients contained significantly more immunoreactivity by RIA and TxB2 EIA (but not by 2,3-dinor TxB2 EIA). Conclusion: RIA and EIA resulted in vastly different absolute values. The difference found between healthy volunteers and asthmatic patients however, suggests an increased level of TxA2 in the airways of asthmatics.

AB - Objective: Differences between detection techniques may be partly responsible for variable mediator concentrations reported in exhaled breath condensate (EBC). We compared two types of immunoassays to estimate thromboxane A2 (TxA2) concentration. Materials and methods: Thromboxane B2 (TxB2) levels were measured by enzyme immunoassay (EIA) and TxB2/2,3-dinor TxB2 by radioimmunoassay (RIA) in 10 healthy subjects and 13 asthmatic patients. 2,3-Dinor TxB2 was also determined by a separate EIA. Results: Thromboxane was detected in all samples by RIA, but only in about 75 % of samples by EIA. 2,3-Dinor TxB2 was detected in most samples. There was no agreement between the results of the different immunoassays. As compared to healthy subjects, exhaled breath condensates of asthmatic patients contained significantly more immunoreactivity by RIA and TxB2 EIA (but not by 2,3-dinor TxB2 EIA). Conclusion: RIA and EIA resulted in vastly different absolute values. The difference found between healthy volunteers and asthmatic patients however, suggests an increased level of TxA2 in the airways of asthmatics.

KW - Asthma

KW - Enzyme immunoassay

KW - Exhaled breath condensate

KW - Radioimmunoassay

KW - Thromboxane A/B

UR - http://www.scopus.com/inward/record.url?scp=24744434955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24744434955&partnerID=8YFLogxK

U2 - 10.1007/s00011-005-1361-x

DO - 10.1007/s00011-005-1361-x

M3 - Article

VL - 54

SP - 350

EP - 355

JO - Inflammation Research

JF - Inflammation Research

SN - 1023-3830

IS - 8

ER -