Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes

Ildikó Takács, Szabolcs Horváth, Ágnes Molnár, Sarolta Gáspár, Rebeka Hajós, Ágnes Meczker, Péter Kóbor, J. Lantos, Szaniszló Jávor, Borbála Balatonyi, György Szekeres, E. Rőth, G. Wéber

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Summary. Intraperitoneal surgical mesh implantation is required for laparoscopic ventral hernia repair. Composite meshes are well known in animal models and human practice. The aim of our study is to compare the biological behaviour of two different textured siliconecovered polypropylene meshes. Transmural abdominal wall defect was created in 40 rabbits and treated as follows: In 20 animals a polypropylene mesh with a laminar silicone covering (LSPP) and in the rest a macroporous textured mesh knitted of silicone-impregnated polypropylene filaments (MSPP) was applied. One and three weeks after implantation we evaluated the intraperitoneal adhesion formation of the mesh macroscopically, histologically and immunohistochemically to detect the reactive cells, especially inflammatory, endothelial and mesothelial cells, as well as their proliferative activity, and with Scanning Electron microscopy to visualize the surface of the meshes. The adhesion formation caused by the composites showed no statistical difference after one week although in the three weeks old samples the LSPP adhesion was significantly weaker than that of MSPP. As complications, serome formation in both groups, fistulas, abscesses, and sc. haematoma in the LSPP group were found. Only in MSPP containing tissues was the decrease of Ki-67 positive proliferating cells significant. A significant increase in VEGF expressing cells was observed only in MSPP containing three week old samples, suggesting better regulation of vascular growth in tissues surrounding the implants. In one week old specimens we observed an irregular proliferation of cytokeratin containing mesothelial cells in both group. The intraperitoneal surface of MSPP mesh was covered with neoperitoneum, while it was not regurarly seen on LSPP mesh after three week.

Original languageEnglish
Pages (from-to)821-830
Number of pages10
JournalHistology and Histopathology
Volume26
Issue number7
Publication statusPublished - Jul 2011

Fingerprint

Surgical Mesh
Polypropylenes
Silicones
Ventral Hernia
Herniorrhaphy
Abdominal Wall
Keratins
Hematoma
Electron Scanning Microscopy
Abscess
Vascular Endothelial Growth Factor A
Fistula
Blood Vessels
Endothelial Cells
Animal Models
Rabbits
Growth

Keywords

  • Cell proliferation
  • Cytokeratin
  • Intraperitoneal adhesions
  • Mesh

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Takács, I., Horváth, S., Molnár, Á., Gáspár, S., Hajós, R., Meczker, Á., ... Wéber, G. (2011). Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes. Histology and Histopathology, 26(7), 821-830.

Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes. / Takács, Ildikó; Horváth, Szabolcs; Molnár, Ágnes; Gáspár, Sarolta; Hajós, Rebeka; Meczker, Ágnes; Kóbor, Péter; Lantos, J.; Jávor, Szaniszló; Balatonyi, Borbála; Szekeres, György; Rőth, E.; Wéber, G.

In: Histology and Histopathology, Vol. 26, No. 7, 07.2011, p. 821-830.

Research output: Contribution to journalArticle

Takács, I, Horváth, S, Molnár, Á, Gáspár, S, Hajós, R, Meczker, Á, Kóbor, P, Lantos, J, Jávor, S, Balatonyi, B, Szekeres, G, Rőth, E & Wéber, G 2011, 'Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes', Histology and Histopathology, vol. 26, no. 7, pp. 821-830.
Takács I, Horváth S, Molnár Á, Gáspár S, Hajós R, Meczker Á et al. Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes. Histology and Histopathology. 2011 Jul;26(7):821-830.
Takács, Ildikó ; Horváth, Szabolcs ; Molnár, Ágnes ; Gáspár, Sarolta ; Hajós, Rebeka ; Meczker, Ágnes ; Kóbor, Péter ; Lantos, J. ; Jávor, Szaniszló ; Balatonyi, Borbála ; Szekeres, György ; Rőth, E. ; Wéber, G. / Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes. In: Histology and Histopathology. 2011 ; Vol. 26, No. 7. pp. 821-830.
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