Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells

Brian Moldt, Csaba Miskey, Nicklas Heine Staunstrup, Andreas Gogol-Döring, Rasmus O. Bak, Nynne Sharma, Lajos Mátés, Zsuzsanna Izsvák, Wei Chen, Zoltán Ivics, Jacob Giehm Mikkelsen

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57 Citations (Scopus)

Abstract

It has been previously shown that integrase-defective HIV-1-based gene vectors can serve, with moderate efficiency, as substrate for DNA transposition by a transiently expressed Sleeping Beauty (SB) transposase. Here, we describe the enhanced gene transfer properties of a HIV-1/SB hybrid vector that allows efficient DNA transposition, facilitated by the hyperactive SB100X transposase, from vector DNA intermediates in primary human cells. Potent transposase-dependent integration of genetic cargo carried by the hybrid HIV-1/SB vector (up to 160-fold above background) is reported in human cell lines as well as in primary human fibroblasts and keratinocytes. The efficiency of transgene integration in context of the newly developed hybrid vector is comparable with that of conventional lentiviral vectors (LVs). Integration profiles of integrating HIV-1-derived vectors and SB transposons mobilized from LVs are investigated by deep sequencing of a large number of integration sites. A significant bias of lentiviral integrations in genes is reported, confirming that biological properties of the viral integration machinery facilitate preferred insertion into actively transcribed genomic regions. In sharp contrast, lentiviral insertions catalyzed by the SB100X transposase are far more random with respect to genes. Based on these properties, HIV-1/SB vectors may become valuable tools for genetic engineering and therapeutic gene transfer.

Original languageEnglish
Pages (from-to)1499-1510
Number of pages12
JournalMolecular Therapy
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 2011

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

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    Moldt, B., Miskey, C., Staunstrup, N. H., Gogol-Döring, A., Bak, R. O., Sharma, N., Mátés, L., Izsvák, Z., Chen, W., Ivics, Z., & Mikkelsen, J. G. (2011). Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells. Molecular Therapy, 19(8), 1499-1510. https://doi.org/10.1038/mt.2011.47