Comparative gene expression profiles of Trichoderma harzianum proteases during in vitro nematode egg-parasitism

Márton Szabó, Péter Urbán, Ferenc Virányi, L. Kredics, C. Fekete

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The parasitism of nematodes by Trichoderma harzianum involves the production and co-ordinated secretion of various eggshell-degrading enzymes. While several protease-encoding genes of Trichoderma spp. have been characterized under different biocontrol conditions, their functional role in the nematode egg-infection process has been poorly understood to date. The present study was carried out to investigate the role and importance of different proteases implicated in nematode egg-parasitism. Parallel expressional characterization of 13 peptidase-encoding genes clearly suggests that the highly transcribed genes encoding acidic serine protease pra1; aspartic protease p6281 and p9438; metalloendopeptidase p7455 and the most prominently induced sedolisin serine protease p5216 were co-expressed and they may play pivotal roles in the egg-parasitic process. In addition, gene expression profiles indicated similarities (e.g. p1324) as well as characteristic differences (e.g. p5216, p7455, p9438, p5431, prb1, SS10 and SA76) between egg-parasitism and other biocontrol-related processes. These results underline the diversity and the functional specialization of fungal protease genes and reveal new insights into the host-pathogen interaction between nematode eggs and T. harzianum.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalBiological Control
Volume67
Issue number3
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Trichoderma harzianum
parasitism
proteinases
Nematoda
gene expression
serine proteinases
biological control
genes
aspartic proteinases
host-pathogen relationships
peptidases
Trichoderma
metalloproteinases
egg shell
secretion
enzymes
infection

Keywords

  • Biological control
  • Caenorhabditis elegans eggs as model system
  • Coordinated gene expression
  • Host-pathogen interaction
  • The role of protease-encoding genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Comparative gene expression profiles of Trichoderma harzianum proteases during in vitro nematode egg-parasitism. / Szabó, Márton; Urbán, Péter; Virányi, Ferenc; Kredics, L.; Fekete, C.

In: Biological Control, Vol. 67, No. 3, 12.2013, p. 337-343.

Research output: Contribution to journalArticle

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