Comparative functional analysis of full-length and N-terminal fragments of phytochrome C, D and E in red light-induced signaling

Éva Ádám, Stefan Kircher, Peng Liu, Zsuzsanna Mérai, Nahuel González-Schain, Maximilian Hörner, András Viczián, Elena Monte, Robert A. Sharrock, Eberhard Schäfer, Ferenc Nagy

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14 Citations (Scopus)

Abstract

Phytochromes (phy) C, D and E are involved in the regulation of red/far-red light-induced photomorphogenesis of Arabidopsis thaliana, but only limited data are available on the mode of action and biological function of these lesser studied phytochrome species. We fused N-terminal fragments or full-length PHYC, D and E to YELLOW FLUORESCENT PROTEIN (YFP), and analyzed the function, stability and intracellular distribution of these fusion proteins in planta. The activity of the constitutively nuclear-localized homodimers of N-terminal fragments was comparable with that of full-length PHYC, D, E-YFP, and resulted in the regulation of various red light-induced photomorphogenic responses in the studied genetic backgrounds. PHYE-YFP was active in the absence of phyB and phyD, and PHYE-YFP controlled responses, as well as accumulation, of the fusion protein in the nuclei, was saturated at low fluence rates of red light and did not require functional FAR-RED ELONGATED HYPOCOTYL1 (FHY-1) and FHY-1-like proteins. Our data suggest that PHYC-YFP, PHYD-YFP and PHYE-YFP fusion proteins, as well as their truncated N-terminal derivatives, are biologically active in the modulation of red light-regulated photomorphogenesis. We propose that PHYE-YFP can function as a homodimer and that low-fluence red light-induced translocation of phyE and phyA into the nuclei is mediated by different molecular mechanisms.

Original languageEnglish
Pages (from-to)86-96
Number of pages11
JournalNew Phytologist
Volume200
Issue number1
DOIs
Publication statusPublished - Oct 1 2013

Keywords

  • Nuclear body formation
  • Nuclear translocation
  • Photomorphogenesis
  • Photoreceptor
  • Phytochrome E

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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    Ádám, É., Kircher, S., Liu, P., Mérai, Z., González-Schain, N., Hörner, M., Viczián, A., Monte, E., Sharrock, R. A., Schäfer, E., & Nagy, F. (2013). Comparative functional analysis of full-length and N-terminal fragments of phytochrome C, D and E in red light-induced signaling. New Phytologist, 200(1), 86-96. https://doi.org/10.1111/nph.12364