Comparative examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP)-Deficient mice

A. Tamás, K. Szabadfi, A. Nemeth, B. Fulop, P. Kiss, T. Atlasz, R. Gábriel, H. Hashimoto, A. Baba, N. Shintani, Z. Helyes, D. Reglodi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide with wellknown neuroprotective and neurotrophic effects. The involvement of PACAP in sensory processing has also been documented, but little is known about its effects in the auditory system. PACAP and its specific receptor (PAC1) are present in the cochlea and in brain structures involved in auditory pathways. Recently, we have shown that PACAP protects cochlear cells against oxidative stressinduced apoptosis. The endolymphatic Ca 2+ concentration controlled by Ca 2+ buffers of the hair cells is essential for the normal hearing processes. In this study we examined the localization of PAC1 receptor and Ca2 + buffering proteins (parvalbumin, calretinin, calbindin) in the inner ear of 5-day-old PACAP-deficient mice compared with wild-type mice in order to get a closer insight into the effect of endogenous PACAP in the cochlear function. We did not find differences in the distribution pattern of PAC1 receptors between the two groups, but wild-type animals showed significantly higher PAC1 receptor expression. In contrast, inner and outer hair cells of PACAP-deficient mice showed more pronounced parvalbumin, calbindin, and calretinin immunopositivity compared with wild-type mice. Elevated endolymphatic Ca2 + is deleterious for cochlear function, while the high concentration of Ca2 + buffers in hair cells may offer protection. The increased immunoreactivity of Ca2 + binding proteins in the absence of PACAP provide further evidence the important role of PACAP in the hearing processes.

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalNeurotoxicity Research
Volume21
Issue number4
DOIs
Publication statusPublished - May 2012

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Inner Ear
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Cochlea
Calbindin 2
Calbindins
Parvalbumins
Cells
Audition
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Hearing
Buffers
Auditory Pathways
Wild Animals
Neuroprotective Agents
Neuropeptides
Brain
Carrier Proteins
Animals

Keywords

  • Calcium binding proteins
  • Cochlea
  • Hair cells
  • Knockout
  • PACAP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Comparative examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP)-Deficient mice. / Tamás, A.; Szabadfi, K.; Nemeth, A.; Fulop, B.; Kiss, P.; Atlasz, T.; Gábriel, R.; Hashimoto, H.; Baba, A.; Shintani, N.; Helyes, Z.; Reglodi, D.

In: Neurotoxicity Research, Vol. 21, No. 4, 05.2012, p. 435-444.

Research output: Contribution to journalArticle

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