Comparative evaluation of the effect of antioxidants in the conservation of ram semen

P. Sarlós, A. Molnár, M. Kókai, Gy Gábor, J. Rátky

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The aim of the present study was to develop a treatment supporting the membrane of ram spermatozoa. Semen of different ejaculates collected from breeding rams was mixed and samples of 109 sperm cells per ml and Tris-egg yolk extender were completed with the following antioxidants: α-tocopherol acetate (E), glutathione peroxidase (GP), Aromex® (AR), resveratrol (R), resveratrol + vitamin E (RE), resveratrol + Aromex® (RAR), resveratrol + GP (RGP). Peroxidation was evaluated by the analysis of malondialdehyde (MDA) during incubation for 30, 60 and 120 rain at 37°C as well as during a 24-h incubation at 5°C. The success of preservation was checked in a 9-day-long period by observing the acrosomal defects and the motility of spermatozoa. Concentration of MDA was 4.06 nmol/109 spermatozoa in samples treated with 15 μg R while the control sample contained 69.79 nmol MDA per 109 spermatozoa after 24-h incubation. Following 30-, 60- and 120-min storage the concentration of MDA in control and R-treated samples was 25.89, 36.91, 49.57 and 3.69, 3.74, 3.74 nmol/109 spermatozoa, respectively. Moreover, a significantly higher proportion of motile sperm cells was observed in the treated than in the control samples. The frequency of acrosomal defects was lower in the treated groups than in the control. These results indicate that RAR treatment can improve the effects of ram semen preservation.

Original languageEnglish
Pages (from-to)235-245
Number of pages11
JournalActa Veterinaria Hungarica
Volume50
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Semen
rams
resveratrol
Spermatozoa
semen
Antioxidants
spermatozoa
antioxidants
malondialdehyde
Malondialdehyde
glutathione peroxidase
Glutathione Peroxidase
sampling
Semen Preservation
peroxidation
Egg Yolk
sperm motility
Rain
egg yolk
tocopherols

Keywords

  • Acrosomal damage
  • Antioxidant
  • Conservation
  • Ram semen
  • Sperm motility

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparative evaluation of the effect of antioxidants in the conservation of ram semen. / Sarlós, P.; Molnár, A.; Kókai, M.; Gábor, Gy; Rátky, J.

In: Acta Veterinaria Hungarica, Vol. 50, No. 2, 2002, p. 235-245.

Research output: Contribution to journalArticle

Sarlós, P. ; Molnár, A. ; Kókai, M. ; Gábor, Gy ; Rátky, J. / Comparative evaluation of the effect of antioxidants in the conservation of ram semen. In: Acta Veterinaria Hungarica. 2002 ; Vol. 50, No. 2. pp. 235-245.
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