Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation

I. Mikó, N. Németh, Viktoria Sogor, Ferenc Kiss, Eniko Toth, Katalin Peto, Andrea Furka, Erzsebet Vanyolos, L. Tóth, J. Varga, Krisztian Szigeti, Ilona Benk, Anna V. Olah, I. Furka

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3 Citations (Scopus)

Abstract

BACKGROUND: Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated. OBJECTIVE: For better evaluation and follow-up of the various spleen-preserving operative techniques' effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model. METHODS: Beagle dogs were subjected to control (n=6), splenectomy (SE, n=4), partial and subtotal spleen resection (n=4/each) or spleen autotransplantation groups (AU, Furka's spleen-chip method, n=8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan). RESULTS: By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups' values were lower versus control and higher than in SE. CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.

Original languageEnglish
Pages (from-to)83-96
Number of pages14
JournalClinical Hemorheology and Microcirculation
Volume66
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

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Erythrocyte Deformability
Autologous Transplantation
Splenectomy
Spleen
Canidae
Theoretical Models
Dogs

Keywords

  • bulk filtrometry
  • red blood cell deformability
  • slit flow and rotational ektacytometry
  • spleen autotransplantation
  • Spleen filtration function
  • spleen partial or subtotal resection
  • splenectomy

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

@article{3684c91b40264461b8eaf9e1eb90d8b0,
title = "Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation",
abstract = "BACKGROUND: Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated. OBJECTIVE: For better evaluation and follow-up of the various spleen-preserving operative techniques' effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model. METHODS: Beagle dogs were subjected to control (n=6), splenectomy (SE, n=4), partial and subtotal spleen resection (n=4/each) or spleen autotransplantation groups (AU, Furka's spleen-chip method, n=8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan). RESULTS: By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups' values were lower versus control and higher than in SE. CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.",
keywords = "bulk filtrometry, red blood cell deformability, slit flow and rotational ektacytometry, spleen autotransplantation, Spleen filtration function, spleen partial or subtotal resection, splenectomy",
author = "I. Mik{\'o} and N. N{\'e}meth and Viktoria Sogor and Ferenc Kiss and Eniko Toth and Katalin Peto and Andrea Furka and Erzsebet Vanyolos and L. T{\'o}th and J. Varga and Krisztian Szigeti and Ilona Benk and Olah, {Anna V.} and I. Furka",
year = "2017",
month = "1",
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doi = "10.3233/CH-160231",
language = "English",
volume = "66",
pages = "83--96",
journal = "Clinical Hemorheology and Microcirculation",
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TY - JOUR

T1 - Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques

T2 - Partial or subtotal spleen resection and spleen autotransplantation

AU - Mikó, I.

AU - Németh, N.

AU - Sogor, Viktoria

AU - Kiss, Ferenc

AU - Toth, Eniko

AU - Peto, Katalin

AU - Furka, Andrea

AU - Vanyolos, Erzsebet

AU - Tóth, L.

AU - Varga, J.

AU - Szigeti, Krisztian

AU - Benk, Ilona

AU - Olah, Anna V.

AU - Furka, I.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - BACKGROUND: Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated. OBJECTIVE: For better evaluation and follow-up of the various spleen-preserving operative techniques' effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model. METHODS: Beagle dogs were subjected to control (n=6), splenectomy (SE, n=4), partial and subtotal spleen resection (n=4/each) or spleen autotransplantation groups (AU, Furka's spleen-chip method, n=8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan). RESULTS: By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups' values were lower versus control and higher than in SE. CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.

AB - BACKGROUND: Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated. OBJECTIVE: For better evaluation and follow-up of the various spleen-preserving operative techniques' effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model. METHODS: Beagle dogs were subjected to control (n=6), splenectomy (SE, n=4), partial and subtotal spleen resection (n=4/each) or spleen autotransplantation groups (AU, Furka's spleen-chip method, n=8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan). RESULTS: By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups' values were lower versus control and higher than in SE. CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.

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KW - red blood cell deformability

KW - slit flow and rotational ektacytometry

KW - spleen autotransplantation

KW - Spleen filtration function

KW - spleen partial or subtotal resection

KW - splenectomy

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