Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride

Krisztina Kovács, G. Szakács, Guido Zacchi

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Cellulase and β-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 °C) and for hydrolysis of SPS (40 °C) than the Rut C30 enzymes (60 °C for FPA and 50 °C for hydrolysis). Due to high levels of extracellular β-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T. atroviride isolates.

Original languageEnglish
Pages (from-to)1350-1357
Number of pages8
JournalBioresource Technology
Volume100
Issue number3
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Enzymatic hydrolysis
Fermentation
fermentation
hydrolysis
Enzymes
enzyme
Steam
Glucosidases
Hydrolysis
filter
Cellulases
Cellulase
Glucose
Assays
glucose
assay
Temperature
temperature

Keywords

  • β-Glucosidase production
  • Cellulase production
  • Enzymatic hydrolysis
  • Trichoderma atroviride
  • Trichoderma reesei

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride. / Kovács, Krisztina; Szakács, G.; Zacchi, Guido.

In: Bioresource Technology, Vol. 100, No. 3, 02.2009, p. 1350-1357.

Research output: Contribution to journalArticle

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