Comparative distribution of VIP in the central nervous system of various species measured by a new radioimmunoassay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vasoactive intestinal polypeptide (VIP) occurs in high concentrations throughout the gut and the nervous system. The presence of VIP has been shown in a number of species, mainly by immunohistochemistry. The aim of the present study was to develop a new, highly specific VIP radioimmunoassay to investigate the distribution of VIP in the central nervous system of various vertebrate and invertebrate species. Different areas of the brain and spinal cord were removed from rats, chickens, turtles, frogs and fishes. The cerebral ganglia and the ventral ganglionic chain were investigated in the earthworm. The tissue samples were processed for VIP radioimmunoassay. Our results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the VIP peptide family. Detection limit of the assay was 0.1 fmol/ml. Highest concentrations were found in the turtle diencephalon, followed by other brain areas in the turtle and rat. All other brain areas in the examined species contained significant levels of VIP. Immunoreactivity was also shown in the cerebral and ventral ganglia of the earthworm. In summary, our results show comparative quantitative distribution in representative species of the phylogenetic line, using the same experimental conditions.

Original languageEnglish
Pages (from-to)3-7
Number of pages5
JournalRegulatory Peptides
Volume109
Issue number1-3
DOIs
Publication statusPublished - Nov 15 2002

Fingerprint

Vasoactive Intestinal Peptide
Neurology
Radioimmunoassay
Central Nervous System
Turtles
Brain
Oligochaeta
Rats
Ganglia
Diencephalon
Fish
Invertebrates
Immune Sera
Assays
Anura
Nervous System
Vertebrates
Limit of Detection
Chickens
Spinal Cord

Keywords

  • Central nervous system
  • Invertebrates
  • Radioimmunoassay
  • Vertebrates
  • VIP

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

@article{22e12530a8e04b4d92fd4a1c48bd34b7,
title = "Comparative distribution of VIP in the central nervous system of various species measured by a new radioimmunoassay",
abstract = "Vasoactive intestinal polypeptide (VIP) occurs in high concentrations throughout the gut and the nervous system. The presence of VIP has been shown in a number of species, mainly by immunohistochemistry. The aim of the present study was to develop a new, highly specific VIP radioimmunoassay to investigate the distribution of VIP in the central nervous system of various vertebrate and invertebrate species. Different areas of the brain and spinal cord were removed from rats, chickens, turtles, frogs and fishes. The cerebral ganglia and the ventral ganglionic chain were investigated in the earthworm. The tissue samples were processed for VIP radioimmunoassay. Our results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the VIP peptide family. Detection limit of the assay was 0.1 fmol/ml. Highest concentrations were found in the turtle diencephalon, followed by other brain areas in the turtle and rat. All other brain areas in the examined species contained significant levels of VIP. Immunoreactivity was also shown in the cerebral and ventral ganglia of the earthworm. In summary, our results show comparative quantitative distribution in representative species of the phylogenetic line, using the same experimental conditions.",
keywords = "Central nervous system, Invertebrates, Radioimmunoassay, Vertebrates, VIP",
author = "J. N{\'e}meth and Bal{\'a}zs Jakab and D. Reglodi and A. Lubics and R. J{\'o}zsa and Tibor Holl{\'o}sy and A. Tam{\'a}s and I. Lengv{\'a}ri and T. G{\"o}rcs and J. Szolcs{\'a}nyi",
year = "2002",
month = "11",
day = "15",
doi = "10.1016/S0167-0115(02)00165-9",
language = "English",
volume = "109",
pages = "3--7",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Comparative distribution of VIP in the central nervous system of various species measured by a new radioimmunoassay

AU - Németh, J.

AU - Jakab, Balázs

AU - Reglodi, D.

AU - Lubics, A.

AU - Józsa, R.

AU - Hollósy, Tibor

AU - Tamás, A.

AU - Lengvári, I.

AU - Görcs, T.

AU - Szolcsányi, J.

PY - 2002/11/15

Y1 - 2002/11/15

N2 - Vasoactive intestinal polypeptide (VIP) occurs in high concentrations throughout the gut and the nervous system. The presence of VIP has been shown in a number of species, mainly by immunohistochemistry. The aim of the present study was to develop a new, highly specific VIP radioimmunoassay to investigate the distribution of VIP in the central nervous system of various vertebrate and invertebrate species. Different areas of the brain and spinal cord were removed from rats, chickens, turtles, frogs and fishes. The cerebral ganglia and the ventral ganglionic chain were investigated in the earthworm. The tissue samples were processed for VIP radioimmunoassay. Our results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the VIP peptide family. Detection limit of the assay was 0.1 fmol/ml. Highest concentrations were found in the turtle diencephalon, followed by other brain areas in the turtle and rat. All other brain areas in the examined species contained significant levels of VIP. Immunoreactivity was also shown in the cerebral and ventral ganglia of the earthworm. In summary, our results show comparative quantitative distribution in representative species of the phylogenetic line, using the same experimental conditions.

AB - Vasoactive intestinal polypeptide (VIP) occurs in high concentrations throughout the gut and the nervous system. The presence of VIP has been shown in a number of species, mainly by immunohistochemistry. The aim of the present study was to develop a new, highly specific VIP radioimmunoassay to investigate the distribution of VIP in the central nervous system of various vertebrate and invertebrate species. Different areas of the brain and spinal cord were removed from rats, chickens, turtles, frogs and fishes. The cerebral ganglia and the ventral ganglionic chain were investigated in the earthworm. The tissue samples were processed for VIP radioimmunoassay. Our results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the VIP peptide family. Detection limit of the assay was 0.1 fmol/ml. Highest concentrations were found in the turtle diencephalon, followed by other brain areas in the turtle and rat. All other brain areas in the examined species contained significant levels of VIP. Immunoreactivity was also shown in the cerebral and ventral ganglia of the earthworm. In summary, our results show comparative quantitative distribution in representative species of the phylogenetic line, using the same experimental conditions.

KW - Central nervous system

KW - Invertebrates

KW - Radioimmunoassay

KW - Vertebrates

KW - VIP

UR - http://www.scopus.com/inward/record.url?scp=0037111264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037111264&partnerID=8YFLogxK

U2 - 10.1016/S0167-0115(02)00165-9

DO - 10.1016/S0167-0115(02)00165-9

M3 - Article

VL - 109

SP - 3

EP - 7

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -