Comparative distribution of somatostatin and thrittene bioactive peptides in the central nervous system of rat measured by radioimmunoassay

G. Fürjes, B. Lelesz, G. K. Tóth, A. Arday, Z. Szilvássy, A. Varga, E. Berényi, J. Németh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present paper the development and application of a novel somatostatin radioimmunoassay are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was separated by reversed-phase HPLC. As a practical application of the novel RIA, the present work measured the somatostatin concentration parallel with the determination of thrittene content in different rat brain areas. The highest somatostatin concentrations were detected in the cerebellum. The thrittene immunoreactivity in the same brain parts showed a similar tendency. In the present study concluded that thrittene is not created via a specific biosynthetical route, but from the pre-prosomatostatin.

Original languageEnglish
Pages (from-to)1741-1749
Number of pages9
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume311
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

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Neurology
Somatostatin
Peptides
Radioimmunoassay
Rats
Brain
Central Nervous System
Labeling
Cerebellum
High Pressure Liquid Chromatography

Keywords

  • Central nervous system (CNS)
  • Iodination
  • Radioimmunoassay (RIA)
  • Somatostatin
  • Thrittene

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Comparative distribution of somatostatin and thrittene bioactive peptides in the central nervous system of rat measured by radioimmunoassay. / Fürjes, G.; Lelesz, B.; Tóth, G. K.; Arday, A.; Szilvássy, Z.; Varga, A.; Berényi, E.; Németh, J.

In: Journal of Radioanalytical and Nuclear Chemistry, Vol. 311, No. 3, 01.03.2017, p. 1741-1749.

Research output: Contribution to journalArticle

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AU - Arday, A.

AU - Szilvássy, Z.

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