Comparative detection of high-risk HPV (16, 18, 33) in cervical bioptic material of county hospital of Tg. Mures.

Z. Pávai, T. Füle, E. Horváth, M. Máthé, Z. Pap, L. Denes, J. Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study was to collect data about the incidence of high-risk HPV (16, 18, 33) types in paraffin embedded cervical bioptic material, including LSIL, HSIL and cervical cancers using immunohistochemistry and nested PCR methods. In our study were included randomly selected 10 LSIL, 18 HSIL and 30 cervical cancer cases. We analyzed the expression of HPV in this specimens with immunohistochemistry used DAKO K1H8 antibody and CHEMICON Mab HPV 16, 16 antibody using LSAB method and Tiramin amplification method, and nested PCR for HPV 16, 18 and 33. In LSIL cases three, in HSIL cases eight and in carcinoma 20 cases were positive for HPV 16 or 18 for immunohistochemistry or PCR. Although this proportion in lower than those reported in the literature, our work signals the existence of the infection in our country and presents a relatively cheap diagnostic method.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalRomanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie
Volume47
Issue number3
Publication statusPublished - 2006

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County Hospitals
Human papillomavirus 18
Human papillomavirus 16
Immunohistochemistry
Uterine Cervical Neoplasms
Polymerase Chain Reaction
Antibodies
Paraffin
Carcinoma
Incidence
Infection

ASJC Scopus subject areas

  • Medicine(all)

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Comparative detection of high-risk HPV (16, 18, 33) in cervical bioptic material of county hospital of Tg. Mures. / Pávai, Z.; Füle, T.; Horváth, E.; Máthé, M.; Pap, Z.; Denes, L.; Jung, J.

In: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, Vol. 47, No. 3, 2006, p. 229-234.

Research output: Contribution to journalArticle

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