Comparative bioanalytical study of 3H-deramciclane in dog plasma. Using a gas chromatography-nitrogen-selective detection (GC-NPD), a new GC-radiochemical detection (GC-RD) and a liquid scintillation method

J. Szúnyog, I. Klebovich, Gy Grézal, I. Hazai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new, highly sensitive and selective gas chromatography method, using radiochemical detection (GC-RD) was developed for the selective determination of 3H-labelled deramciclane and its N-desmethyl metabolite in dog plasma. Inter-day accuracy and precision, as well as system suitability of the GC-RD method was investigated during the method validation. The calibration curve was proved to be linear (r = 0.9986) in a wide concentration range (13-1000 ngeqv mL-1) The lower limit of quantitation (LLOQ) was 13.7 ngeqv mL-1, and the limit of the detection (LOD) was 1 ngeqv mL-1. Using this new GC-RD method, plasma levels of 3H-labelled deramciclane and its metabolite were determined in dogs, after the administration of a single 10 mg kg-1 oral dose. Pharmacokinetic curves and the calculated pharmacokinetic parameters were compared to these obtained using a previously elaborated gas chromatography-nitrogen selective detection method (GC-NPD) and to those obtained by measuring the plasma level of total radioactivity (liquid scintillation counting, LSC). Pharmacokinetic curves and the calculated pharmacokinetic parameters obtained with the two different gas chromatography detection methods (NPD and RD) showed good correlation. Comparison of these results to those acquired by total radioactivity measurement demonstrated that deramciclane was intensively metabolised. Moreover, the biological half-life (t( 1/4 )(β)) of the unknown metabolites proved to be more than a magnitude longer than the half-life of the parent compound or that of N-desmethyl metabolite.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalChromatographia
Volume48
Issue number1-2
DOIs
Publication statusPublished - 1998

Fingerprint

Pharmacokinetics
Scintillation
Metabolites
Gas chromatography
Gas Chromatography
Nitrogen
Dogs
Plasmas
Liquids
Radioactivity measurement
Radioactivity
Half-Life
Scintillation Counting
Calibration
Limit of Detection
deramciclane
neodymium pyrocatechin disulfonate

Keywords

  • H-Deramciclane
  • H-N-desmetil deramciclane metabolite
  • Gas chromatography-nitrogen-selectiv detection (GC-NPD)
  • Gas chromatography-radiochemical detection (GC-RD)
  • Liquid scintillation counting (LSC)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

@article{f0128169fa4f407f995a94347eb94281,
title = "Comparative bioanalytical study of 3H-deramciclane in dog plasma. Using a gas chromatography-nitrogen-selective detection (GC-NPD), a new GC-radiochemical detection (GC-RD) and a liquid scintillation method",
abstract = "A new, highly sensitive and selective gas chromatography method, using radiochemical detection (GC-RD) was developed for the selective determination of 3H-labelled deramciclane and its N-desmethyl metabolite in dog plasma. Inter-day accuracy and precision, as well as system suitability of the GC-RD method was investigated during the method validation. The calibration curve was proved to be linear (r = 0.9986) in a wide concentration range (13-1000 ngeqv mL-1) The lower limit of quantitation (LLOQ) was 13.7 ngeqv mL-1, and the limit of the detection (LOD) was 1 ngeqv mL-1. Using this new GC-RD method, plasma levels of 3H-labelled deramciclane and its metabolite were determined in dogs, after the administration of a single 10 mg kg-1 oral dose. Pharmacokinetic curves and the calculated pharmacokinetic parameters were compared to these obtained using a previously elaborated gas chromatography-nitrogen selective detection method (GC-NPD) and to those obtained by measuring the plasma level of total radioactivity (liquid scintillation counting, LSC). Pharmacokinetic curves and the calculated pharmacokinetic parameters obtained with the two different gas chromatography detection methods (NPD and RD) showed good correlation. Comparison of these results to those acquired by total radioactivity measurement demonstrated that deramciclane was intensively metabolised. Moreover, the biological half-life (t( 1/4 )(β)) of the unknown metabolites proved to be more than a magnitude longer than the half-life of the parent compound or that of N-desmethyl metabolite.",
keywords = "H-Deramciclane, H-N-desmetil deramciclane metabolite, Gas chromatography-nitrogen-selectiv detection (GC-NPD), Gas chromatography-radiochemical detection (GC-RD), Liquid scintillation counting (LSC)",
author = "J. Sz{\'u}nyog and I. Klebovich and Gy Gr{\'e}zal and I. Hazai",
year = "1998",
doi = "10.1007/BF02467529",
language = "English",
volume = "48",
pages = "133--139",
journal = "Chromatographia",
issn = "0009-5893",
publisher = "Springer Vieweg",
number = "1-2",

}

TY - JOUR

T1 - Comparative bioanalytical study of 3H-deramciclane in dog plasma. Using a gas chromatography-nitrogen-selective detection (GC-NPD), a new GC-radiochemical detection (GC-RD) and a liquid scintillation method

AU - Szúnyog, J.

AU - Klebovich, I.

AU - Grézal, Gy

AU - Hazai, I.

PY - 1998

Y1 - 1998

N2 - A new, highly sensitive and selective gas chromatography method, using radiochemical detection (GC-RD) was developed for the selective determination of 3H-labelled deramciclane and its N-desmethyl metabolite in dog plasma. Inter-day accuracy and precision, as well as system suitability of the GC-RD method was investigated during the method validation. The calibration curve was proved to be linear (r = 0.9986) in a wide concentration range (13-1000 ngeqv mL-1) The lower limit of quantitation (LLOQ) was 13.7 ngeqv mL-1, and the limit of the detection (LOD) was 1 ngeqv mL-1. Using this new GC-RD method, plasma levels of 3H-labelled deramciclane and its metabolite were determined in dogs, after the administration of a single 10 mg kg-1 oral dose. Pharmacokinetic curves and the calculated pharmacokinetic parameters were compared to these obtained using a previously elaborated gas chromatography-nitrogen selective detection method (GC-NPD) and to those obtained by measuring the plasma level of total radioactivity (liquid scintillation counting, LSC). Pharmacokinetic curves and the calculated pharmacokinetic parameters obtained with the two different gas chromatography detection methods (NPD and RD) showed good correlation. Comparison of these results to those acquired by total radioactivity measurement demonstrated that deramciclane was intensively metabolised. Moreover, the biological half-life (t( 1/4 )(β)) of the unknown metabolites proved to be more than a magnitude longer than the half-life of the parent compound or that of N-desmethyl metabolite.

AB - A new, highly sensitive and selective gas chromatography method, using radiochemical detection (GC-RD) was developed for the selective determination of 3H-labelled deramciclane and its N-desmethyl metabolite in dog plasma. Inter-day accuracy and precision, as well as system suitability of the GC-RD method was investigated during the method validation. The calibration curve was proved to be linear (r = 0.9986) in a wide concentration range (13-1000 ngeqv mL-1) The lower limit of quantitation (LLOQ) was 13.7 ngeqv mL-1, and the limit of the detection (LOD) was 1 ngeqv mL-1. Using this new GC-RD method, plasma levels of 3H-labelled deramciclane and its metabolite were determined in dogs, after the administration of a single 10 mg kg-1 oral dose. Pharmacokinetic curves and the calculated pharmacokinetic parameters were compared to these obtained using a previously elaborated gas chromatography-nitrogen selective detection method (GC-NPD) and to those obtained by measuring the plasma level of total radioactivity (liquid scintillation counting, LSC). Pharmacokinetic curves and the calculated pharmacokinetic parameters obtained with the two different gas chromatography detection methods (NPD and RD) showed good correlation. Comparison of these results to those acquired by total radioactivity measurement demonstrated that deramciclane was intensively metabolised. Moreover, the biological half-life (t( 1/4 )(β)) of the unknown metabolites proved to be more than a magnitude longer than the half-life of the parent compound or that of N-desmethyl metabolite.

KW - H-Deramciclane

KW - H-N-desmetil deramciclane metabolite

KW - Gas chromatography-nitrogen-selectiv detection (GC-NPD)

KW - Gas chromatography-radiochemical detection (GC-RD)

KW - Liquid scintillation counting (LSC)

UR - http://www.scopus.com/inward/record.url?scp=0031850269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031850269&partnerID=8YFLogxK

U2 - 10.1007/BF02467529

DO - 10.1007/BF02467529

M3 - Article

AN - SCOPUS:0031850269

VL - 48

SP - 133

EP - 139

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - 1-2

ER -