Comparative analysis of the underground parts of Gentiana species by HPLC with diode-array and mass spectrometric detection

Z. Szucs, B. Dános, Sz Nyiredy

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31 Citations (Scopus)

Abstract

The aim of this work was to compare the chemical composition of the underground parts (roots and rhizomes) of Gentiana cruciota L., Gentiana pneumonanthe L., and Gentiana asclepiadea L. - the three gentians native to Hungary - with that of the widely used stomochic Gentiana lutea L., to determine which of the three Hungarian species could be used as a substitute for Gentiana lutea in pharmaceutical preparations. The four gentians were compared by means of RPHPLC with diode-array detection (DAD) and electrospray ionization mass spectrometric detection (ESI-MSD). The quantities of the lead compounds, the secoiridoid-glycosides, in 220 samples of the underground ports of gentians originating from several locations in Hungary, were determined by a more economical RPHPLC-DAD method. The occurrence of the characteristic compounds investigated - bitter principles and xanthones - in the underground parts of the species Gentiana asclepiadea L. suggest it might be a potential replacement for Gentiana lutea L. in pharmaceutical products.

Original languageEnglish
Pages (from-to)S19-S23
JournalChromatographia
Volume56
Issue numberSUPPL.
Publication statusPublished - Jul 25 2002

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Keywords

  • Bitter principles
  • Column liquid chromatography-DAD-MSD
  • Electrospray ionization
  • Gentiana species
  • Xanthones

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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