Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup

Kata Horváti, Bernadett Bacsa, Tamás Mlinkó, Nóra Szabó, F. Hudecz, F. Zsila, Sz. Bősze

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cationic peptides proved fundamental importance as pharmaceutical agents and/or drug carrier moieties functioning in cellular processes. The comparison of the in vitro activity of these peptides is an experimental challenge and a combination of different methods, such as cytotoxicity, internalisation rate, haemolytic and antibacterial effect, is necessary. At the same time, several issues need to be addressed as the assay conditions have a great influence on the measured biological effects and the experimental setup needs to be optimised. Therefore, critical comparison of results from different assays using representative examples of cell penetrating and antimicrobial peptides was performed and optimal test conditions were suggested. Our main goal was to identify carrier peptides for drug delivery systems of antimicrobial drug candidates. Based on the results of internalisation, haemolytic, cytotoxic and antibacterial activity assays, a classification of cationic peptides is advocated. We found eight promising carrier peptides with good penetration ability of which Penetratin, Tat, Buforin and Dhvar4 peptides showed low adverse haemolytic effect. Penetratin, Transportan, Dhvar4 and the hybrid CM15 peptide had the most potent antibacterial activity on Streptococcus pneumoniae (MIC lower than 1.2 μM) and Transportan was effective against Mycobacterium tuberculosis as well. The most selective peptide was the Penetratin, where the effective antimicrobial concentration on pneumococcus was more than 250 times lower than the HC50 value. Therefore, these peptides and their analogues will be further investigated as drug delivery systems for antimicrobial agents.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalAmino Acids
DOIs
Publication statusAccepted/In press - Mar 17 2017

Fingerprint

Membranes
Peptides
Assays
Drug Delivery Systems
Streptococcus pneumoniae
Cell-Penetrating Peptides
Drug Carriers
Anti-Infective Agents
Mycobacterium tuberculosis
Pharmaceutical Preparations
Cytotoxicity
penetratin
transportan

Keywords

  • Antibacterial drug carrier
  • Antimicrobial peptide
  • Cationic peptides
  • Cell penetrating peptide
  • Haemolysis
  • Tuberculosis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides : aspects of experimental setup. / Horváti, Kata; Bacsa, Bernadett; Mlinkó, Tamás; Szabó, Nóra; Hudecz, F.; Zsila, F.; Bősze, Sz.

In: Amino Acids, 17.03.2017, p. 1-15.

Research output: Contribution to journalArticle

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