Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters

Csaba Lantos, Lajos Bóna, K. Boda, János Pauk

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two haploid induction media (190-0 and W14mi) were tested in isolated microspore culture of two triticale (X Triticosecale Wittmack) genotypes. The W14mi medium proved superior for the production of green plantlets in both genotypes. This basic medium (W14) was used to compare two doubled haploid production methods (isolated microspore culture and anther culture) with the same genotypes. The induction of androgenesis was more effective in isolated microspore culture than in anther culture. The number of embryo-like structures was 9.2 times higher in microspore culture (511.0/100 anthers) compared to anther culture (55.5/100 anthers) and the number of regenerant plantlets was also 3.4 times higher (anther culture-20.15/100 anthers; isolated microspore culture-67.6/100 anthers). However, the regenerant plantlets from isolated microspore culture were mainly albinos while predominantly green plantlets were regenerated from anther culture. The production of green plantlets from anther culture (16.8/100 anthers) was 2.9 times higher than from isolated microspore culture (5.8/100 anthers). The efficiency of anther culture was tested with eight winter triticale genotypes. The phenomenon of albinism did not hinder the green plant production in anther culture. Mean green plantlet production was 10.87/100 anthers. This value was two times higher than the number of albinos (5.01/100 anthers) and higher than previously published reports. The anther culture protocol described in this study is an efficient tool for the production of microspore-derived green plantlets in triticale.

Original languageEnglish
Pages (from-to)27-37
Number of pages11
JournalEuphytica
Volume197
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Triticosecale
anther culture
microspores
hexaploidy
anthers
Genotype
plantlets
Haploidy
Albinism
Viridiplantae
albino
genotype
Embryonic Structures
Triticale
In Vitro Techniques
androgenesis
doubled haploids
embryo (plant)
haploidy

Keywords

  • Androgenesis
  • Anther culture
  • Haploid
  • Microspore culture
  • Triticale
  • X Triticosecale Wittmack

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Horticulture
  • Plant Science

Cite this

Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters. / Lantos, Csaba; Bóna, Lajos; Boda, K.; Pauk, János.

In: Euphytica, Vol. 197, No. 1, 2014, p. 27-37.

Research output: Contribution to journalArticle

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