Comparative analysis of a conserved gene block from the genome of the members of the genus ictalurivirus

Andor Doszpoly, M. Benkó, Giuseppe Bovo, Scott E. Lapatra, B. Harrach

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: Partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (HVs). Acipenserid (Aci) HV-2, originating from the white sturgeon (Acipenser transmontanus), and ictalurid (Ic) HV-2, isolated from the black bullhead (Ameiurus melas), are recently approved species of the genus Ictalurivirus of the family Alloherpesviridae. Methods: An almost 8,000-base-pair fragment, spanning between the genes of the DNA polymerase and the ATPase subunit of the terminase, was sequenced from each virus. Results: The size, position and orientation of 2 partial and 3 full open reading frames, contained in the studied genome fragment, proved to be similar to their counterparts in IcHV-1, the type species of the genus Ictalurivirus. Thus, a well-conserved genus-specific gene block was identified. In the members of two other genera (Cyprinivirus and Batrachovirus) of the family Alloherpesviridae, no such gene block could be found; the location and orientation of the homologous genes showed significant divergence. Conclusion: The results of phylogenetic calculations were in good agreement with the genome arrangements inasmuch as AciHV-2, IcHV-1 and -2 are monophyletic and separated from the lineages of the other two genera. The new sequence enabled the inclusion of a hitherto unassigned HV, that of the Australian pilchard, into a phylogenetic calculation.

Original languageEnglish
Pages (from-to)282-289
Number of pages8
JournalIntervirology
Volume54
Issue number5
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Ictalurivirus
Herpesviridae
Genome
Genes
Ictaluridae
DNA-Directed DNA Polymerase
Base Pairing
Open Reading Frames
Adenosine Triphosphatases
Fishes
Viruses

Keywords

  • Alloherpesviridae
  • Black bullhead
  • Fish herpesvirus
  • Gene block
  • Ictalurivirus
  • Sturgeon
  • Subfamily

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Comparative analysis of a conserved gene block from the genome of the members of the genus ictalurivirus. / Doszpoly, Andor; Benkó, M.; Bovo, Giuseppe; Lapatra, Scott E.; Harrach, B.

In: Intervirology, Vol. 54, No. 5, 08.2011, p. 282-289.

Research output: Contribution to journalArticle

Doszpoly, Andor ; Benkó, M. ; Bovo, Giuseppe ; Lapatra, Scott E. ; Harrach, B. / Comparative analysis of a conserved gene block from the genome of the members of the genus ictalurivirus. In: Intervirology. 2011 ; Vol. 54, No. 5. pp. 282-289.
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