Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP

C. V. Nielsen, M. Køie, C. Székely, K. Buchmann

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda). It is not clear if isolates from unrelated hosts represent the same species. Therefore a molecular study was conducted to reveal differences at the DNA level between these isolates. PCR was successfully conducted on three different isolates of Myxobolus aeglefini sampled from cod (Gadus morhua), plaice and dab respectively, using 18S rDNA as template. A PCR product of approx. 1600 base pairs was obtained and RFLP (Restriction Fragment Length Polymerase) was conducted on the fragment with the restriction enzymes Hinf I, Msp I and Hae III. No differences between the isolates were found, suggesting that the three isolates represent the same species.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalBulletin of the European Association of Fish Pathologists
Volume22
Issue number3
Publication statusPublished - 2002

Fingerprint

Myxobolus
Gadus morhua
Pleuronectes
Flounder
Gadiformes
Pleuronectes platessa
cod (fish)
rRNA Genes
ribosomal RNA
parasites
Polymerase Chain Reaction
gene
Pleuronectiformes
Parasites
cartilage
Flatfishes
parasite
genes
Ribosomal DNA
Base Pairing

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

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title = "Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP",
abstract = "The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda). It is not clear if isolates from unrelated hosts represent the same species. Therefore a molecular study was conducted to reveal differences at the DNA level between these isolates. PCR was successfully conducted on three different isolates of Myxobolus aeglefini sampled from cod (Gadus morhua), plaice and dab respectively, using 18S rDNA as template. A PCR product of approx. 1600 base pairs was obtained and RFLP (Restriction Fragment Length Polymerase) was conducted on the fragment with the restriction enzymes Hinf I, Msp I and Hae III. No differences between the isolates were found, suggesting that the three isolates represent the same species.",
author = "Nielsen, {C. V.} and M. K{\o}ie and C. Sz{\'e}kely and K. Buchmann",
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T1 - Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP

AU - Nielsen, C. V.

AU - Køie, M.

AU - Székely, C.

AU - Buchmann, K.

PY - 2002

Y1 - 2002

N2 - The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda). It is not clear if isolates from unrelated hosts represent the same species. Therefore a molecular study was conducted to reveal differences at the DNA level between these isolates. PCR was successfully conducted on three different isolates of Myxobolus aeglefini sampled from cod (Gadus morhua), plaice and dab respectively, using 18S rDNA as template. A PCR product of approx. 1600 base pairs was obtained and RFLP (Restriction Fragment Length Polymerase) was conducted on the fragment with the restriction enzymes Hinf I, Msp I and Hae III. No differences between the isolates were found, suggesting that the three isolates represent the same species.

AB - The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda). It is not clear if isolates from unrelated hosts represent the same species. Therefore a molecular study was conducted to reveal differences at the DNA level between these isolates. PCR was successfully conducted on three different isolates of Myxobolus aeglefini sampled from cod (Gadus morhua), plaice and dab respectively, using 18S rDNA as template. A PCR product of approx. 1600 base pairs was obtained and RFLP (Restriction Fragment Length Polymerase) was conducted on the fragment with the restriction enzymes Hinf I, Msp I and Hae III. No differences between the isolates were found, suggesting that the three isolates represent the same species.

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