Compactin production studies using penicillium brevicompactum under solid-state fermentation conditions

N. S. Shaligram, S. K. Singh, R. S. Singhal, A. Pandey, G. Szakacs

Research output: Contribution to journalArticle

15 Citations (Scopus)


In the present study, compactin production by Penicillium brevicompactum WA 2315 was optimized using solid-state fermentation. The initial one factor at a time approach resulted in improved compactin production of 905 μg gds -1 compared to initial 450 μg gds-1. Subsequently, nutritional, physiological, and biological parameters were screened using fractional factorial and Box-Behnken design. The fractional factorial design studied inoculum age, inoculum volume, pH, NaCl, NH4NO3, MgSO4, and KH2PO4. All parameters were found to be significant except pH and KH2PO4. The Box-Behnken design studied inoculum volume, inoculum age, glycerol, and NH 4NO3 at three different levels. Inoculum volume (p = 0.0013) and glycerol (p = 0.0001) were significant factors with greater effect on response. The interaction effects were not significant. The validation study using model-defined conditions resulted in an improved yield of 1,250 μg gds-1 compactin. Further improvement in yield was obtained using fed batch mode of carbon supplementation. The feeding of glycerol (20% v/v) on day 3 resulted in further improved compactin yield of 1,406 μg gds-1. The present study demonstrates that agro-industrial residues can be successfully used for compactin production, and statistical experiment designs provide an easy tool to improve the process conditions for secondary metabolite production.

Original languageEnglish
Pages (from-to)505-520
Number of pages16
JournalApplied Biochemistry and Biotechnology
Issue number2
Publication statusPublished - Nov 1 2009


  • Box-Behnken design
  • Compactin
  • Fractional factorial design
  • Penicillium brevicompactum WA 2315
  • Solid-state fermentation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Compactin production studies using penicillium brevicompactum under solid-state fermentation conditions'. Together they form a unique fingerprint.

  • Cite this