Common pathway of chromosome condensation in mammalian cells

Gaspar Banfalvi, Gabor Nagy, Mariann Gacsi, Tamas Roszer, Alexei G. Basnakian

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Chromatin folding in the interphase nucleus is not known. We compared the pattern of chromatin condensation in Indian muntjac, Chinese hamster ovary, murine preB, and K562 human erythroleukemia cells during the cell cycle. Fluorescent microscopy showed that chromosome condensation follows a general pathway. Synchronized cells were reversibly permeabilized and used to isolate interphase chromatin structures. Based on their structures two major categories of intermediates were distinguished: (1) decondensed chromatin and (2) condensed chromosomal forms. (1) Chromatin forms were found between the G1 and mid-S phase involving veil-like, supercoiled, fibrous, ribboned structures; (2) condensing chromosomal forms appeared in the late-S, G2, and M phase, including strings, chromatin bodies, elongated prechromosomes, precondensed chromosomes, and metaphase chromosomes. Results demonstrate that interphase chromosomes are clustered in domains; condensing interphase chromosomes are linearly arranged. Our results raise questions related to telomer sequences and to the chemical nature of chromosome connectivity.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalDNA and Cell Biology
Volume25
Issue number5
DOIs
Publication statusPublished - May 2006

Fingerprint

Chromatin
Chromosomes
Interphase
S Phase
Muntjacs
Leukemia, Erythroblastic, Acute
G2 Phase
Metaphase
Cricetulus
Cell Division
Microscopy
Ovary
Cell Cycle

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Common pathway of chromosome condensation in mammalian cells. / Banfalvi, Gaspar; Nagy, Gabor; Gacsi, Mariann; Roszer, Tamas; Basnakian, Alexei G.

In: DNA and Cell Biology, Vol. 25, No. 5, 05.2006, p. 295-301.

Research output: Contribution to journalArticle

Banfalvi, G, Nagy, G, Gacsi, M, Roszer, T & Basnakian, AG 2006, 'Common pathway of chromosome condensation in mammalian cells', DNA and Cell Biology, vol. 25, no. 5, pp. 295-301. https://doi.org/10.1089/dna.2006.25.295
Banfalvi, Gaspar ; Nagy, Gabor ; Gacsi, Mariann ; Roszer, Tamas ; Basnakian, Alexei G. / Common pathway of chromosome condensation in mammalian cells. In: DNA and Cell Biology. 2006 ; Vol. 25, No. 5. pp. 295-301.
@article{92bf7bd07cf4411ca7e6670f244c5a61,
title = "Common pathway of chromosome condensation in mammalian cells",
abstract = "Chromatin folding in the interphase nucleus is not known. We compared the pattern of chromatin condensation in Indian muntjac, Chinese hamster ovary, murine preB, and K562 human erythroleukemia cells during the cell cycle. Fluorescent microscopy showed that chromosome condensation follows a general pathway. Synchronized cells were reversibly permeabilized and used to isolate interphase chromatin structures. Based on their structures two major categories of intermediates were distinguished: (1) decondensed chromatin and (2) condensed chromosomal forms. (1) Chromatin forms were found between the G1 and mid-S phase involving veil-like, supercoiled, fibrous, ribboned structures; (2) condensing chromosomal forms appeared in the late-S, G2, and M phase, including strings, chromatin bodies, elongated prechromosomes, precondensed chromosomes, and metaphase chromosomes. Results demonstrate that interphase chromosomes are clustered in domains; condensing interphase chromosomes are linearly arranged. Our results raise questions related to telomer sequences and to the chemical nature of chromosome connectivity.",
author = "Gaspar Banfalvi and Gabor Nagy and Mariann Gacsi and Tamas Roszer and Basnakian, {Alexei G.}",
year = "2006",
month = "5",
doi = "10.1089/dna.2006.25.295",
language = "English",
volume = "25",
pages = "295--301",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Common pathway of chromosome condensation in mammalian cells

AU - Banfalvi, Gaspar

AU - Nagy, Gabor

AU - Gacsi, Mariann

AU - Roszer, Tamas

AU - Basnakian, Alexei G.

PY - 2006/5

Y1 - 2006/5

N2 - Chromatin folding in the interphase nucleus is not known. We compared the pattern of chromatin condensation in Indian muntjac, Chinese hamster ovary, murine preB, and K562 human erythroleukemia cells during the cell cycle. Fluorescent microscopy showed that chromosome condensation follows a general pathway. Synchronized cells were reversibly permeabilized and used to isolate interphase chromatin structures. Based on their structures two major categories of intermediates were distinguished: (1) decondensed chromatin and (2) condensed chromosomal forms. (1) Chromatin forms were found between the G1 and mid-S phase involving veil-like, supercoiled, fibrous, ribboned structures; (2) condensing chromosomal forms appeared in the late-S, G2, and M phase, including strings, chromatin bodies, elongated prechromosomes, precondensed chromosomes, and metaphase chromosomes. Results demonstrate that interphase chromosomes are clustered in domains; condensing interphase chromosomes are linearly arranged. Our results raise questions related to telomer sequences and to the chemical nature of chromosome connectivity.

AB - Chromatin folding in the interphase nucleus is not known. We compared the pattern of chromatin condensation in Indian muntjac, Chinese hamster ovary, murine preB, and K562 human erythroleukemia cells during the cell cycle. Fluorescent microscopy showed that chromosome condensation follows a general pathway. Synchronized cells were reversibly permeabilized and used to isolate interphase chromatin structures. Based on their structures two major categories of intermediates were distinguished: (1) decondensed chromatin and (2) condensed chromosomal forms. (1) Chromatin forms were found between the G1 and mid-S phase involving veil-like, supercoiled, fibrous, ribboned structures; (2) condensing chromosomal forms appeared in the late-S, G2, and M phase, including strings, chromatin bodies, elongated prechromosomes, precondensed chromosomes, and metaphase chromosomes. Results demonstrate that interphase chromosomes are clustered in domains; condensing interphase chromosomes are linearly arranged. Our results raise questions related to telomer sequences and to the chemical nature of chromosome connectivity.

UR - http://www.scopus.com/inward/record.url?scp=33744794924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744794924&partnerID=8YFLogxK

U2 - 10.1089/dna.2006.25.295

DO - 10.1089/dna.2006.25.295

M3 - Article

C2 - 16716119

AN - SCOPUS:33744794924

VL - 25

SP - 295

EP - 301

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 5

ER -