Commercial strain-derived clinical Saccharomyces cerevisiae can evolve new phenotypes without higher pathogenicity

Walter P. Pfliegler, Enikő Boros, Kitti Pázmándi, Ágnes Jakab, Imre Zsuga, Renátó Kovács, E. Urbán, Zsuzsa Antunovics, Attila Bácsi, M. Sipiczki, László Majoros, I. Pócsi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Scope: Saccharomyces cerevisiae is one of the most important microbes in food industry, but there is growing evidence on its potential pathogenicity as well. Its status as a member of human mycobiome is still not fully understood. Methods and results: In this study, we characterize clinical S. cerevisiae isolates from Hungarian hospitals along with commercial baking and probiotic strains, and determine their phenotypic parameters, virulence factors, interactions with human macrophages, and pathogenicity. Four of the clinical isolates could be traced back to commercial strains based on genetic fingerprinting. Our observations indicate that the commercial-derived clinical isolates have evolved new phenotypes and show similar, or in two cases, significantly decreased pathogenicity. Furthermore, immunological experiments revealed that the variability in human primary macrophage activation after coincubation with yeasts is largely donor and not isolate dependent. Conclusion: Isolates in this study offer an interesting insight into the potential microevolution of probiotic and food strains in human hosts. These commensal yeasts display various changes in their phenotypes, indicating that the colonization of the host does not necessarily impose a selective pressure toward higher virulence/pathogenicity.

Original languageEnglish
Article number1601099
JournalMolecular Nutrition and Food Research
Volume61
Issue number11
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Virulence
Saccharomyces cerevisiae
pathogenicity
Phenotype
phenotype
probiotics
Probiotics
virulence
yeasts
macrophage activation
Yeasts
baking
DNA fingerprinting
DNA Fingerprinting
Macrophage Activation
Food Industry
food industry
Virulence Factors
macrophages
microorganisms

Keywords

  • Baking yeast
  • Commensal
  • Microevolution
  • Mycobiom
  • Probiotic

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Commercial strain-derived clinical Saccharomyces cerevisiae can evolve new phenotypes without higher pathogenicity. / Pfliegler, Walter P.; Boros, Enikő; Pázmándi, Kitti; Jakab, Ágnes; Zsuga, Imre; Kovács, Renátó; Urbán, E.; Antunovics, Zsuzsa; Bácsi, Attila; Sipiczki, M.; Majoros, László; Pócsi, I.

In: Molecular Nutrition and Food Research, Vol. 61, No. 11, 1601099, 01.11.2017.

Research output: Contribution to journalArticle

Pfliegler, Walter P. ; Boros, Enikő ; Pázmándi, Kitti ; Jakab, Ágnes ; Zsuga, Imre ; Kovács, Renátó ; Urbán, E. ; Antunovics, Zsuzsa ; Bácsi, Attila ; Sipiczki, M. ; Majoros, László ; Pócsi, I. / Commercial strain-derived clinical Saccharomyces cerevisiae can evolve new phenotypes without higher pathogenicity. In: Molecular Nutrition and Food Research. 2017 ; Vol. 61, No. 11.
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