Combined physiological-anatomical approaches to study lateral inhibition

Z. Kisvárday, John M. Crook, P. Buzás, Ulf T. Eysel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In the visual cortex, large basket cells form the cellular basis of long-range lateral inhibition. The present paper focuses on combinations of methods with which large basket cells can be studied in the context of extensive neuronal representations. In the first approach, the topographic relationship between large basket axons and known functional representations such as orientation, direction, and ocular dominance is analysed. Functional mapping is carried out using extracellular electrode recordings or optical imaging of intrinsic signals followed by 3-dimensional anatomical reconstruction of biocytin stained large basket cells in the same regions. In the second approach, the contribution of lateral inhibition to orientation and direction selectivity is assessed using the GABA inactivation paradigm and direct inhibitory projections from the inactivation to recording sites are demonstrated with biocytin staining and injections of [3H]nipecotic acid, a radioactive marker for GABAergic cells. The limitation of these approaches is that they can only be used in cortical regions which lie on the surface of the brain. (C) 2000 Published by Elsevier Science B.V.

Original languageEnglish
Pages (from-to)91-106
Number of pages16
JournalJournal of Neuroscience Methods
Volume103
Issue number1
DOIs
Publication statusPublished - Nov 15 2000

Fingerprint

ocular Dominance
Optical Imaging
Visual Cortex
gamma-Aminobutyric Acid
Axons
Electrodes
Staining and Labeling
Injections
Brain
Direction compound
biocytin
nipecotic acid

Keywords

  • Biocytin
  • Electrophysiology
  • Functional maps
  • GABA
  • Large basket cells
  • Nipecotic acid
  • Optical imaging
  • Visual cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Combined physiological-anatomical approaches to study lateral inhibition. / Kisvárday, Z.; Crook, John M.; Buzás, P.; Eysel, Ulf T.

In: Journal of Neuroscience Methods, Vol. 103, No. 1, 15.11.2000, p. 91-106.

Research output: Contribution to journalArticle

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