Combined in vivo recording of neural signals and iontophoretic injection of pathway tracers using a hollow silicon microelectrode

Z. Fekete, E. Pálfi, G. Márton, M. Handbauer, Zs Bérces, I. Ulbert, A. Pongrácz, L. Négyessy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This paper presents the results of in vivo local release of a neuronal tracer, biotinylated dextran amine (BDA) in the rat somatosensory cortex using monolithically integrated microfluidic channel of a silicon neural microelectrode. The tracer injection is controlled by iontophoresis using Pt electrodes in the vicinity of the outlet of the microfluidic channel. Using 3-5. μA, 5-7. s on/off cycle and 15-20. min total injection time the localized injection resulted in clear anterograde and retrograde BDA labeling both within the cortex and in subcortical structures. Anterograde and retrograde labeling revealed the fine details of neuronal processes including dendritic spines and axon terminal-like endings. Injection sites appeared clear lacking any strong diffuse background labeling. Electrophysiological recording performed with the same microdevice immediately after the iontophoresis indicated normal cortical functioning. The results prove that the combination of in vivo multichannel neural recording and controlled tracer injection using a single implanted microdevice is feasible, and therefore it can be a powerful tool for studying the connectome of the brain.

Original languageEnglish
JournalSensors and Actuators, B: Chemical
DOIs
Publication statusAccepted/In press - Oct 2 2015

Fingerprint

Microelectrodes
Silicon
Labeling
tracers
hollow
Dextran
recording
injection
Microfluidics
Amines
marking
silicon
dextrans
cortexes
amines
Rats
Brain
axons
spine
outlets

Keywords

  • Biotinylated dextran amine
  • Microiontophoresis
  • Neural microelectrode
  • Neuroanatomical tracer injection
  • Neuronal labeling

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

Combined in vivo recording of neural signals and iontophoretic injection of pathway tracers using a hollow silicon microelectrode. / Fekete, Z.; Pálfi, E.; Márton, G.; Handbauer, M.; Bérces, Zs; Ulbert, I.; Pongrácz, A.; Négyessy, L.

In: Sensors and Actuators, B: Chemical, 02.10.2015.

Research output: Contribution to journalArticle

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AU - Bérces, Zs

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