Combined genotyping strategy reveals structural differences between Aspergillus flavus lineages from different habitats impacting human health

Nikolett Baranyi, Sándor Kocsubé, Daniela Jakšić Despot, Maja Šegvić Klarić, András Szekeres, Ottó Bencsik, Anita Kecskeméti, Palanisamy Manikandan, Beáta Tóth, László Kredics, Jamal M. Khaled, Naiyf S. Alharbi, Csaba Vágvölgyi, János Varga

Research output: Contribution to journalArticle

Abstract

Aspergillus flavus is a filamentous fungus which is widespread on agricultural products and also able to cause various human diseases. This species is frequently isolated from indoor air as well, furthermore, it is known as a common causal agent of keratomycosis, particularly in subtropical and tropical areas. It is also able to produce aflatoxins, one of the most carcinogenic mycotoxins which are harmful to animals and humans. In this study, 59 A. flavus isolates from four different habitats and 1 A. minisclerotigenes isolate were investigated. The isolates were identified and confirmed at the species level by the sequence analysis of a part of their calmodulin gene. Applying a combined analysis of UP-PCR, microsatellite, and calmodulin sequence data, the four group of isolates formed separate clusters on the phylogenetic tree. Examining the distribution of mating type genes MAT1-1 and MAT1-2, a ratio of approximately 3:1 was determined, and no correlation was found between the carried mating type gene and the aflatoxin production capability. HPLC analysis revealed that none of the examined isolates collected from indoor air or maize in Central Europe were able to produce aflatoxins, while about half of the isolates from India produced these mycotoxins under the test conditions.

Original languageEnglish
Pages (from-to)899-909
Number of pages11
JournalJournal of basic microbiology
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 2017

Keywords

  • Aspergillus flavus
  • UP-PCR analysis
  • aflatoxins
  • mating type genes
  • microsatellite analysis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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    Baranyi, N., Kocsubé, S., Jakšić Despot, D., Šegvić Klarić, M., Szekeres, A., Bencsik, O., Kecskeméti, A., Manikandan, P., Tóth, B., Kredics, L., Khaled, J. M., Alharbi, N. S., Vágvölgyi, C., & Varga, J. (2017). Combined genotyping strategy reveals structural differences between Aspergillus flavus lineages from different habitats impacting human health. Journal of basic microbiology, 57(11), 899-909. https://doi.org/10.1002/jobm.201700243