Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats - I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance

L. Vígh, I. Horváth, J. Woltjes, T. Farkas, P. van Hassett, P. J C Kuiper

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalPlanta
Volume170
Issue number1
DOIs
Publication statusPublished - Jan 1987

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frost resistance
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
X-ray diffraction
X-Ray Diffraction
Triticum
Phospholipids
phospholipids
wheat
cultivars
Lipids
Freezing
Fatty Acids
Triticum aestivum
Gels
Temperature
Wounds and Injuries
plant damage
lipids
frost

Keywords

  • Fatty acid composition
  • Frost tolerance
  • Membrane fluidity
  • Phase transition temperature
  • Triticum (frost hardiness)

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats - I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance",
abstract = "Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.",
keywords = "Fatty acid composition, Frost tolerance, Membrane fluidity, Phase transition temperature, Triticum (frost hardiness)",
author = "L. V{\'i}gh and I. Horv{\'a}th and J. Woltjes and T. Farkas and {van Hassett}, P. and Kuiper, {P. J C}",
year = "1987",
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T1 - Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats - I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance

AU - Vígh, L.

AU - Horváth, I.

AU - Woltjes, J.

AU - Farkas, T.

AU - van Hassett, P.

AU - Kuiper, P. J C

PY - 1987/1

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N2 - Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.

AB - Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.

KW - Fatty acid composition

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KW - Membrane fluidity

KW - Phase transition temperature

KW - Triticum (frost hardiness)

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