Combined atomic force microscopy and fluorescence microscopy.

Research output: Contribution to journalArticle

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Abstract

The atomic force microscope (AFM) is a high-resolution scanning-probe instrument which has become an important tool for cellular and molecular biophysics in recent years, but lacks the time resolution and functional specificities offered by fluorescence microscopic techniques. The advantages of both methods may be exploited by combining and synchronizing them. In this paper, the biological applications of AFM, fluorescence, and their combinations are briefly reviewed, and the assembly and utilization of a spatially and temporally synchronized AFM and total internal reflection fluorescence microscope are described. The application of the method is demonstrated on a fluorescently labeled cell culture.

Original languageEnglish
Pages (from-to)439-456
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume736
DOIs
Publication statusPublished - 2011

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Atomic Force Microscopy
Fluorescence Microscopy
Fluorescence
Biophysics
Cell Culture Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Combined atomic force microscopy and fluorescence microscopy. / Kellermayer, M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 736, 2011, p. 439-456.

Research output: Contribution to journalArticle

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