Combinatorial natural products: From cloning to analysis

Paul Budworth, Julia Khandurina, A. Guttman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Medicinal compounds from plants represent one of the largest and most diverse groups of plant secondary metabolites. The advent of advanced bioinformatics tools and modern genetic technology allowed for manipulation of biosynthetic pathways with the potential of generating novel chemical entities. First, public databases of secondary metabolite related enzymes were interrogated to identify relevant plant genes from vinca rosea (Catharanthus roseus) and other species. Genes of interest were tested after cloning by transfection into tobacco cell cultures using DNA viral vectors. The biosynthetic enzymes coded by these genes were over-expressed in the host. Automated solvent extraction procedure was employed to extract secondary metabolites from plant leaf tissues and transfected tobacco cell culture samples. The composition of the extracts was analyzed by state of the art bioanalytical methods such as high performance liquid chromatography and capillary electrophoresis to monitor changes in secondary metabolite patterns.

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalCurrent Medicinal Chemistry
Volume12
Issue number6
Publication statusPublished - 2005

Fingerprint

Catharanthus
Cloning
Metabolites
Biological Products
Tobacco
Organism Cloning
Cell Culture Techniques
Plant Genes
Plant Leaves
Genes
Biosynthetic Pathways
Viral DNA
Capillary Electrophoresis
Enzymes
Medicinal Plants
Computational Biology
Cell culture
Transfection
High Pressure Liquid Chromatography
Databases

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

Combinatorial natural products : From cloning to analysis. / Budworth, Paul; Khandurina, Julia; Guttman, A.

In: Current Medicinal Chemistry, Vol. 12, No. 6, 2005, p. 703-711.

Research output: Contribution to journalArticle

Budworth, P, Khandurina, J & Guttman, A 2005, 'Combinatorial natural products: From cloning to analysis', Current Medicinal Chemistry, vol. 12, no. 6, pp. 703-711.
Budworth, Paul ; Khandurina, Julia ; Guttman, A. / Combinatorial natural products : From cloning to analysis. In: Current Medicinal Chemistry. 2005 ; Vol. 12, No. 6. pp. 703-711.
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