Folyadekkromatografias modszerek kombinalt alkalmazasa a magas alkaloid tartalmu makfajtak nemesiteseben

Translated title of the contribution: Combinations of liquid chromatographic methods for the breeding of high alkaloid content poppy

S. Fater, Z. Samu, M. Szatmary, S. Nyiredy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An analytical strategy for the determination of alkaloid content of poppy capsules was developed for the efficient plant breeding. The first step of the strategy is a screening by circular multilayer (ML) over pressured layer chromatography (OPLC) with large number (28 samples/chromatoplate) of samples under UV (254 and 366 nm) light. The next step is a TLC separation with densitometric evaluation for quantitative determination of the selected samples (ca. 50% of all samples). These results are confirmed by ionpair reversed-phase (RP) high performance liquid chromatography (HPLC) for the most interesting (20% of all samples) poppy capsules. If the difference of the two applied quantitative methods is higher than 10% the investigations have to be repeated by new mobile phase systems with different solvent strenght and selectivity factor values to eliminate the disturbing effect of the minor compounds which appear during the plant breeding. On the basis of the analytical results two new populations were formed, one of them ('MAMOR') has high morfine (~20 mg/g) content, the other one ('TEMAX') is characterized by ability of tebain (~16 mg/g) accumulation. The two new poppy sorts are verification for the effectiveness of the combination of different separation techniques (OPLC, TLC, HPLC) and UV detection modes (off-line and on-line) for medicinal plant breeding.

Original languageHungarian
Pages (from-to)211-219
Number of pages9
JournalActa Pharmaceutica Hungarica
Volume67
Issue number5
Publication statusPublished - 1997

Fingerprint

Papaver
Alkaloids
Breeding
Capsules
Chromatography
High Pressure Liquid Chromatography
Reverse-Phase Chromatography
Medicinal Plants
Light
Population
Plant Breeding

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Folyadekkromatografias modszerek kombinalt alkalmazasa a magas alkaloid tartalmu makfajtak nemesiteseben. / Fater, S.; Samu, Z.; Szatmary, M.; Nyiredy, S.

In: Acta Pharmaceutica Hungarica, Vol. 67, No. 5, 1997, p. 211-219.

Research output: Contribution to journalArticle

@article{4255355b8771488e8834cca800eafb87,
title = "Folyadekkromatografias modszerek kombinalt alkalmazasa a magas alkaloid tartalmu makfajtak nemesiteseben",
abstract = "An analytical strategy for the determination of alkaloid content of poppy capsules was developed for the efficient plant breeding. The first step of the strategy is a screening by circular multilayer (ML) over pressured layer chromatography (OPLC) with large number (28 samples/chromatoplate) of samples under UV (254 and 366 nm) light. The next step is a TLC separation with densitometric evaluation for quantitative determination of the selected samples (ca. 50{\%} of all samples). These results are confirmed by ionpair reversed-phase (RP) high performance liquid chromatography (HPLC) for the most interesting (20{\%} of all samples) poppy capsules. If the difference of the two applied quantitative methods is higher than 10{\%} the investigations have to be repeated by new mobile phase systems with different solvent strenght and selectivity factor values to eliminate the disturbing effect of the minor compounds which appear during the plant breeding. On the basis of the analytical results two new populations were formed, one of them ('MAMOR') has high morfine (~20 mg/g) content, the other one ('TEMAX') is characterized by ability of tebain (~16 mg/g) accumulation. The two new poppy sorts are verification for the effectiveness of the combination of different separation techniques (OPLC, TLC, HPLC) and UV detection modes (off-line and on-line) for medicinal plant breeding.",
author = "S. Fater and Z. Samu and M. Szatmary and S. Nyiredy",
year = "1997",
language = "Hungarian",
volume = "67",
pages = "211--219",
journal = "Acta Pharmaceutica Hungarica",
issn = "0001-6659",
publisher = "Magyar Gyogyszereszeti Tarsasag",
number = "5",

}

TY - JOUR

T1 - Folyadekkromatografias modszerek kombinalt alkalmazasa a magas alkaloid tartalmu makfajtak nemesiteseben

AU - Fater, S.

AU - Samu, Z.

AU - Szatmary, M.

AU - Nyiredy, S.

PY - 1997

Y1 - 1997

N2 - An analytical strategy for the determination of alkaloid content of poppy capsules was developed for the efficient plant breeding. The first step of the strategy is a screening by circular multilayer (ML) over pressured layer chromatography (OPLC) with large number (28 samples/chromatoplate) of samples under UV (254 and 366 nm) light. The next step is a TLC separation with densitometric evaluation for quantitative determination of the selected samples (ca. 50% of all samples). These results are confirmed by ionpair reversed-phase (RP) high performance liquid chromatography (HPLC) for the most interesting (20% of all samples) poppy capsules. If the difference of the two applied quantitative methods is higher than 10% the investigations have to be repeated by new mobile phase systems with different solvent strenght and selectivity factor values to eliminate the disturbing effect of the minor compounds which appear during the plant breeding. On the basis of the analytical results two new populations were formed, one of them ('MAMOR') has high morfine (~20 mg/g) content, the other one ('TEMAX') is characterized by ability of tebain (~16 mg/g) accumulation. The two new poppy sorts are verification for the effectiveness of the combination of different separation techniques (OPLC, TLC, HPLC) and UV detection modes (off-line and on-line) for medicinal plant breeding.

AB - An analytical strategy for the determination of alkaloid content of poppy capsules was developed for the efficient plant breeding. The first step of the strategy is a screening by circular multilayer (ML) over pressured layer chromatography (OPLC) with large number (28 samples/chromatoplate) of samples under UV (254 and 366 nm) light. The next step is a TLC separation with densitometric evaluation for quantitative determination of the selected samples (ca. 50% of all samples). These results are confirmed by ionpair reversed-phase (RP) high performance liquid chromatography (HPLC) for the most interesting (20% of all samples) poppy capsules. If the difference of the two applied quantitative methods is higher than 10% the investigations have to be repeated by new mobile phase systems with different solvent strenght and selectivity factor values to eliminate the disturbing effect of the minor compounds which appear during the plant breeding. On the basis of the analytical results two new populations were formed, one of them ('MAMOR') has high morfine (~20 mg/g) content, the other one ('TEMAX') is characterized by ability of tebain (~16 mg/g) accumulation. The two new poppy sorts are verification for the effectiveness of the combination of different separation techniques (OPLC, TLC, HPLC) and UV detection modes (off-line and on-line) for medicinal plant breeding.

UR - http://www.scopus.com/inward/record.url?scp=0031218789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031218789&partnerID=8YFLogxK

M3 - Article

VL - 67

SP - 211

EP - 219

JO - Acta Pharmaceutica Hungarica

JF - Acta Pharmaceutica Hungarica

SN - 0001-6659

IS - 5

ER -