Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

Eszter Molnár, Soujanya Kuntam, Pradeep Kumar Reddy Cingaram, Begüm Peksel, Bhavyashree Suresh, Gabriella Fábián, Liliána Z. Fehér, Attila Bokros, Ágnes Medgyesi, F. Ayaydin, L. Puskás

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment- specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1-10 micromolar range, they were non-toxic even during long-term incubations.

Original languageEnglish
Pages (from-to)9999-10013
Number of pages15
JournalMolecules
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Confocal microscopy
staining
Microarrays
Fluorescent Dyes
Confocal Microscopy
dyes
Staining and Labeling
microscopy
Molecules
compartments
cells
Cell Membrane
High-Throughput Screening Assays
molecules
screening
microscopes
Cell membranes
Cytotoxicity
membranes
fluorescence

Keywords

  • Confocal laser scanning microscopy
  • Fluorescent dye discovery
  • High-throughput screening
  • Small molecule microarray

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery. / Molnár, Eszter; Kuntam, Soujanya; Cingaram, Pradeep Kumar Reddy; Peksel, Begüm; Suresh, Bhavyashree; Fábián, Gabriella; Fehér, Liliána Z.; Bokros, Attila; Medgyesi, Ágnes; Ayaydin, F.; Puskás, L.

In: Molecules, Vol. 18, No. 8, 08.2013, p. 9999-10013.

Research output: Contribution to journalArticle

Molnár, E, Kuntam, S, Cingaram, PKR, Peksel, B, Suresh, B, Fábián, G, Fehér, LZ, Bokros, A, Medgyesi, Á, Ayaydin, F & Puskás, L 2013, 'Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery', Molecules, vol. 18, no. 8, pp. 9999-10013. https://doi.org/10.3390/molecules18089999
Molnár, Eszter ; Kuntam, Soujanya ; Cingaram, Pradeep Kumar Reddy ; Peksel, Begüm ; Suresh, Bhavyashree ; Fábián, Gabriella ; Fehér, Liliána Z. ; Bokros, Attila ; Medgyesi, Ágnes ; Ayaydin, F. ; Puskás, L. / Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery. In: Molecules. 2013 ; Vol. 18, No. 8. pp. 9999-10013.
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