Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

Eszter Molnár, Soujanya Kuntam, Pradeep Kumar Reddy Cingaram, Begüm Peksel, Bhavyashree Suresh, Gabriella Fábián, Liliána Z. Fehér, Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, László G. Puskás

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Abstract

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment- specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1-10 micromolar range, they were non-toxic even during long-term incubations.

Original languageEnglish
Pages (from-to)9999-10013
Number of pages15
JournalMolecules
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 1 2013

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Keywords

  • Confocal laser scanning microscopy
  • Fluorescent dye discovery
  • High-throughput screening
  • Small molecule microarray

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Molnár, E., Kuntam, S., Cingaram, P. K. R., Peksel, B., Suresh, B., Fábián, G., Fehér, L. Z., Bokros, A., Medgyesi, Á., Ayaydin, F., & Puskás, L. G. (2013). Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery. Molecules, 18(8), 9999-10013. https://doi.org/10.3390/molecules18089999