Combination of MALDI-TOF MS and PBP2′ latex agglutination assay for rapid MRSA detection

Marianna Ábrók, Andrea Lázár, Mária Szécsényi, Judit Deák, E. Urbán

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Sample preparation was optimized for MALDI-TOF MS directly from the selective enrichment broth to detect Staphylococcus aureus. A combination of MALDI-TOF MS and the PBP2′ latex agglutination assay was applied for MRSA screening and evaluated on 255 clinical samples. MRSA colonisation can be reported already 18–24 h after sample collection.

Original languageEnglish
Pages (from-to)122-124
Number of pages3
JournalJournal of Microbiological Methods
Volume144
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Agglutination
Latex
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus

Keywords

  • Latex agglutination test
  • Methicillin-resistant Staphylococcus aureus
  • Penicillin-binding protein 2′
  • Salt-containing trypticase soy broth
  • Sample preparation
  • Screening

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Combination of MALDI-TOF MS and PBP2′ latex agglutination assay for rapid MRSA detection. / Ábrók, Marianna; Lázár, Andrea; Szécsényi, Mária; Deák, Judit; Urbán, E.

In: Journal of Microbiological Methods, Vol. 144, 01.01.2018, p. 122-124.

Research output: Contribution to journalArticle

Ábrók, Marianna ; Lázár, Andrea ; Szécsényi, Mária ; Deák, Judit ; Urbán, E. / Combination of MALDI-TOF MS and PBP2′ latex agglutination assay for rapid MRSA detection. In: Journal of Microbiological Methods. 2018 ; Vol. 144. pp. 122-124.
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