Combination of cobalt labelling with immunocytochemical reactions for electron microscopic investigations on frog spinal cord

I. Nagy, A. Sik, E. Polgar, M. Petkó, M. Antal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase-antiperoxidase pre-embedding or immunogold post-embedding immunocytochemical labelling. Our results have shown that cobalt labelling can easily be distinguished from both of the immunoreaction end products. The protocol of cobalt labelling did not affect the immunoreactivity of structures. The morphology and synaptology of cobalt labelled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling techniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron microscopic studies on nervous tissues.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalMicroscopy Research and Technique
Volume28
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

frogs
spinal cord
cobalt
Cobalt
Anura
Labeling
marking
Spinal Cord
electrons
Electrons
labeling techniques
embedding
Nerve Tissue
Peroxidase
staining
peroxidase
Staining and Labeling
Tissue
products
profiles

Keywords

  • Calcitonin gene-related peptide
  • Double labelling
  • Gamma-amino butyric acid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

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AU - Nagy, I.

AU - Sik, A.

AU - Polgar, E.

AU - Petkó, M.

AU - Antal, M.

PY - 1994

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AB - Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase-antiperoxidase pre-embedding or immunogold post-embedding immunocytochemical labelling. Our results have shown that cobalt labelling can easily be distinguished from both of the immunoreaction end products. The protocol of cobalt labelling did not affect the immunoreactivity of structures. The morphology and synaptology of cobalt labelled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling techniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron microscopic studies on nervous tissues.

KW - Calcitonin gene-related peptide

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KW - Gamma-amino butyric acid

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