Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking water

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Aims: In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human pathogenic Cryptosporidium parvum, Cryptosporidium hominis, Cryptosporidium meleagridis and Giardia duodenalis Assemblage A and B specific DNA sequence. Methods and Results: During the evaluation of the new concentration and detection technique, spiked reagent and matrix water samples plus blank samples were filtered and tested. In total, 27 samples have been investigated. The results clearly demonstrate that the methodology of using a new ARAD filter, which passed through 1000 l of drinking water with high turbidity (2 NTU), and followed by the LAMP assay was able to detect at least one (oo)cyst in 10 l of drinking water based on a 1000-l sample, taken over a 24-h period. Conclusions: The described protozoa detection methodology is sensitive, rapid and cost-effective. Significance and Impact of the Study: This effective procedure will be useful for small waterworks to achieve continuous monitoring and is also of value for screening catchments to identify those that require further treatment and more detailed microscopic counts.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalLetters in Applied Microbiology
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Giardia lamblia
Cryptosporidium
Drinking Water
Cysts
Costs and Cost Analysis
Cryptosporidium parvum
Giardia
Oocysts
Water

Keywords

  • Detection
  • Drinking water
  • Environmental health
  • Protozoa
  • Rapid techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

@article{df75b314703f4f128a603a9b2a2bc6b1,
title = "Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking water",
abstract = "Aims: In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human pathogenic Cryptosporidium parvum, Cryptosporidium hominis, Cryptosporidium meleagridis and Giardia duodenalis Assemblage A and B specific DNA sequence. Methods and Results: During the evaluation of the new concentration and detection technique, spiked reagent and matrix water samples plus blank samples were filtered and tested. In total, 27 samples have been investigated. The results clearly demonstrate that the methodology of using a new ARAD filter, which passed through 1000 l of drinking water with high turbidity (2 NTU), and followed by the LAMP assay was able to detect at least one (oo)cyst in 10 l of drinking water based on a 1000-l sample, taken over a 24-h period. Conclusions: The described protozoa detection methodology is sensitive, rapid and cost-effective. Significance and Impact of the Study: This effective procedure will be useful for small waterworks to achieve continuous monitoring and is also of value for screening catchments to identify those that require further treatment and more detailed microscopic counts.",
keywords = "Detection, Drinking water, Environmental health, Protozoa, Rapid techniques",
author = "J. Plutzer and A. T{\"o}r{\"o}kn{\'e} and P. Karanis",
year = "2010",
month = "1",
doi = "10.1111/j.1472-765X.2009.02758.x",
language = "English",
volume = "50",
pages = "82--88",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking water

AU - Plutzer, J.

AU - Törökné, A.

AU - Karanis, P.

PY - 2010/1

Y1 - 2010/1

N2 - Aims: In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human pathogenic Cryptosporidium parvum, Cryptosporidium hominis, Cryptosporidium meleagridis and Giardia duodenalis Assemblage A and B specific DNA sequence. Methods and Results: During the evaluation of the new concentration and detection technique, spiked reagent and matrix water samples plus blank samples were filtered and tested. In total, 27 samples have been investigated. The results clearly demonstrate that the methodology of using a new ARAD filter, which passed through 1000 l of drinking water with high turbidity (2 NTU), and followed by the LAMP assay was able to detect at least one (oo)cyst in 10 l of drinking water based on a 1000-l sample, taken over a 24-h period. Conclusions: The described protozoa detection methodology is sensitive, rapid and cost-effective. Significance and Impact of the Study: This effective procedure will be useful for small waterworks to achieve continuous monitoring and is also of value for screening catchments to identify those that require further treatment and more detailed microscopic counts.

AB - Aims: In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human pathogenic Cryptosporidium parvum, Cryptosporidium hominis, Cryptosporidium meleagridis and Giardia duodenalis Assemblage A and B specific DNA sequence. Methods and Results: During the evaluation of the new concentration and detection technique, spiked reagent and matrix water samples plus blank samples were filtered and tested. In total, 27 samples have been investigated. The results clearly demonstrate that the methodology of using a new ARAD filter, which passed through 1000 l of drinking water with high turbidity (2 NTU), and followed by the LAMP assay was able to detect at least one (oo)cyst in 10 l of drinking water based on a 1000-l sample, taken over a 24-h period. Conclusions: The described protozoa detection methodology is sensitive, rapid and cost-effective. Significance and Impact of the Study: This effective procedure will be useful for small waterworks to achieve continuous monitoring and is also of value for screening catchments to identify those that require further treatment and more detailed microscopic counts.

KW - Detection

KW - Drinking water

KW - Environmental health

KW - Protozoa

KW - Rapid techniques

UR - http://www.scopus.com/inward/record.url?scp=72149084641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72149084641&partnerID=8YFLogxK

U2 - 10.1111/j.1472-765X.2009.02758.x

DO - 10.1111/j.1472-765X.2009.02758.x

M3 - Article

C2 - 19895420

AN - SCOPUS:72149084641

VL - 50

SP - 82

EP - 88

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 1

ER -