Colour pigments of Trichoderma harzianum: Preliminary investigations with thin-layer chromatography-Fourier transform infrared spectroscopy and high-performance liquid chromatography with diode array and mass spectrometric detection

Gergely Csiktusnádi Kiss, E. Forgács, T. Cserháti, Juan Antonio Vizcaino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The colour pigments of Trichoderma harzianum fermentation broth were separated and the main fractions were tentatively identified by reversed-phase thin-layer chromatography-Fourier transform infrared spectroscopy (RP-TLC-FT-IR), RP-HPLC-diode array detection and RP-HPLC-MS. It was established that the multistep gradient elution developed for RP-TLC separation of pigments can be successfully used as a pilot method for the rational design of gradient elution in RP-HPLC for the separation of the same pigments. FT-IR and MS measurements were unable to identify the exact chemical structures of the main pigment fractions, the presence of OH, = CH and C=O (RP-TLC-FT-IR) and OH and NH2 substructures (RP-HPLC-MS) was confirmed. It was assumed that the main pigment fractions are oxidation polymers originating from monomer molecules containing polar substructures and double bonds in the alkyl chain which are liable for oxidation during the aerobic fermentation process. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalJournal of Chromatography A
Volume896
Issue number1-2
DOIs
Publication statusPublished - Oct 27 2000

Fingerprint

Thin layer chromatography
Trichoderma
High performance liquid chromatography
Fourier Transform Infrared Spectroscopy
Thin Layer Chromatography
Pigments
Diodes
Color
High Pressure Liquid Chromatography
Fermentation
Reverse-Phase Chromatography
Oxidation
Polymers
Monomers
Molecules

Keywords

  • Pigments
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Colour pigments of Trichoderma harzianum: Preliminary investigations with thin-layer chromatography-Fourier transform infrared spectroscopy and high-performance liquid chromatography with diode array and mass spectrometric detection",
abstract = "The colour pigments of Trichoderma harzianum fermentation broth were separated and the main fractions were tentatively identified by reversed-phase thin-layer chromatography-Fourier transform infrared spectroscopy (RP-TLC-FT-IR), RP-HPLC-diode array detection and RP-HPLC-MS. It was established that the multistep gradient elution developed for RP-TLC separation of pigments can be successfully used as a pilot method for the rational design of gradient elution in RP-HPLC for the separation of the same pigments. FT-IR and MS measurements were unable to identify the exact chemical structures of the main pigment fractions, the presence of OH, = CH and C=O (RP-TLC-FT-IR) and OH and NH2 substructures (RP-HPLC-MS) was confirmed. It was assumed that the main pigment fractions are oxidation polymers originating from monomer molecules containing polar substructures and double bonds in the alkyl chain which are liable for oxidation during the aerobic fermentation process. (C) 2000 Elsevier Science B.V.",
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AU - Csiktusnádi Kiss, Gergely

AU - Forgács, E.

AU - Cserháti, T.

AU - Vizcaino, Juan Antonio

PY - 2000/10/27

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N2 - The colour pigments of Trichoderma harzianum fermentation broth were separated and the main fractions were tentatively identified by reversed-phase thin-layer chromatography-Fourier transform infrared spectroscopy (RP-TLC-FT-IR), RP-HPLC-diode array detection and RP-HPLC-MS. It was established that the multistep gradient elution developed for RP-TLC separation of pigments can be successfully used as a pilot method for the rational design of gradient elution in RP-HPLC for the separation of the same pigments. FT-IR and MS measurements were unable to identify the exact chemical structures of the main pigment fractions, the presence of OH, = CH and C=O (RP-TLC-FT-IR) and OH and NH2 substructures (RP-HPLC-MS) was confirmed. It was assumed that the main pigment fractions are oxidation polymers originating from monomer molecules containing polar substructures and double bonds in the alkyl chain which are liable for oxidation during the aerobic fermentation process. (C) 2000 Elsevier Science B.V.

AB - The colour pigments of Trichoderma harzianum fermentation broth were separated and the main fractions were tentatively identified by reversed-phase thin-layer chromatography-Fourier transform infrared spectroscopy (RP-TLC-FT-IR), RP-HPLC-diode array detection and RP-HPLC-MS. It was established that the multistep gradient elution developed for RP-TLC separation of pigments can be successfully used as a pilot method for the rational design of gradient elution in RP-HPLC for the separation of the same pigments. FT-IR and MS measurements were unable to identify the exact chemical structures of the main pigment fractions, the presence of OH, = CH and C=O (RP-TLC-FT-IR) and OH and NH2 substructures (RP-HPLC-MS) was confirmed. It was assumed that the main pigment fractions are oxidation polymers originating from monomer molecules containing polar substructures and double bonds in the alkyl chain which are liable for oxidation during the aerobic fermentation process. (C) 2000 Elsevier Science B.V.

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