Colocalization of substance P with tumor necrosis factor-α in the lymphocytes and mast cells in gastritis in experimental rats

Éva Pongor, Károly Altdorfer, E. Fehér

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Substance P (SP) elicits numerous potent neuroimmunomodulatory effects, increasing the release of tumor necrosis factor alpha (TNF-α). The study aimed to investigate immunoneural communication in experimentally-induced gastritis in rats. Methods: SP-containing nerve fibers and lymphocytes and mast cells were counted in the mucosa of the stomachs of rats using double immunohistochemical and confocal laser microscopic methods, proving colocalization of SP and TNF-α in the lymphocytes and mast cells. Results: In controls, only the nerve fibers showed SP immunoreactivity (IR). However, in gastritis the number of SP-IR fibers and TNF-α IR lymphocytes and mast cells increased significantly (P <0.001); SP-IR fibers were seen in close contact with lymphocytes and mast cells. Numerous lymphocytes (13.1%) and mast cells (10.8%) showed IR for both SP and TNF-α (colocalization) within the same cells. Conclusion: SP release from nerve fibers, lymphocytes and mast cells together with TNF-α can enhance the development of gastric inflammation and participate in tissue damage in gastritis.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalInflammation Research
Volume60
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Gastritis
Substance P
Mast Cells
Tumor Necrosis Factor-alpha
Lymphocytes
Nerve Fibers
Stomach
Mucous Membrane
Lasers
Communication
Inflammation
Neurons

Keywords

  • Colocalization
  • Gastritis
  • Lymphocytes
  • Mast cells
  • Substance P
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

Colocalization of substance P with tumor necrosis factor-α in the lymphocytes and mast cells in gastritis in experimental rats. / Pongor, Éva; Altdorfer, Károly; Fehér, E.

In: Inflammation Research, Vol. 60, No. 2, 02.2011, p. 163-168.

Research output: Contribution to journalArticle

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N2 - Objective: Substance P (SP) elicits numerous potent neuroimmunomodulatory effects, increasing the release of tumor necrosis factor alpha (TNF-α). The study aimed to investigate immunoneural communication in experimentally-induced gastritis in rats. Methods: SP-containing nerve fibers and lymphocytes and mast cells were counted in the mucosa of the stomachs of rats using double immunohistochemical and confocal laser microscopic methods, proving colocalization of SP and TNF-α in the lymphocytes and mast cells. Results: In controls, only the nerve fibers showed SP immunoreactivity (IR). However, in gastritis the number of SP-IR fibers and TNF-α IR lymphocytes and mast cells increased significantly (P <0.001); SP-IR fibers were seen in close contact with lymphocytes and mast cells. Numerous lymphocytes (13.1%) and mast cells (10.8%) showed IR for both SP and TNF-α (colocalization) within the same cells. Conclusion: SP release from nerve fibers, lymphocytes and mast cells together with TNF-α can enhance the development of gastric inflammation and participate in tissue damage in gastritis.

AB - Objective: Substance P (SP) elicits numerous potent neuroimmunomodulatory effects, increasing the release of tumor necrosis factor alpha (TNF-α). The study aimed to investigate immunoneural communication in experimentally-induced gastritis in rats. Methods: SP-containing nerve fibers and lymphocytes and mast cells were counted in the mucosa of the stomachs of rats using double immunohistochemical and confocal laser microscopic methods, proving colocalization of SP and TNF-α in the lymphocytes and mast cells. Results: In controls, only the nerve fibers showed SP immunoreactivity (IR). However, in gastritis the number of SP-IR fibers and TNF-α IR lymphocytes and mast cells increased significantly (P <0.001); SP-IR fibers were seen in close contact with lymphocytes and mast cells. Numerous lymphocytes (13.1%) and mast cells (10.8%) showed IR for both SP and TNF-α (colocalization) within the same cells. Conclusion: SP release from nerve fibers, lymphocytes and mast cells together with TNF-α can enhance the development of gastric inflammation and participate in tissue damage in gastritis.

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