Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells

Sudha Warrier Mitra, Éva Mezey, B. Hunyady, LaShawn Chamberlain, Edward Hayes, Forrest Foor, Yining Wang, Agnes Schonbrunn, James M. Schaeffer

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic δ-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenger RNA for both sst2 and sst5 have been reported in the rat pancreas, and the sst2A receptor protein has been localized to rat pancreatic α and pancreatic polypeptide-secreting cells in the islets as well as to pancreatic acinar cells. In this study we have used double immunostaining to show that the sst5 protein is expressed exclusively in the β-cells of rat pancreatic islets and localizes with insulin-secreting α-cells. The sst5 receptor is not colocalized with sst2A. Thus, in the rat SRIF inhibits pancreatic insulin and glucagon secretion via different sst receptor subtypes.

Original languageEnglish
Pages (from-to)3790-3796
Number of pages7
JournalEndocrinology
Volume140
Issue number8
Publication statusPublished - 1999

Fingerprint

Somatostatin Receptors
Somatostatin
Insulin
Glucagon
Pancreatic Polypeptide-Secreting Cells
Acinar Cells
Insulin-Secreting Cells
G-Protein-Coupled Receptors
Islets of Langerhans
Pancreas
Proteins
Messenger RNA
Genes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Mitra, S. W., Mezey, É., Hunyady, B., Chamberlain, L., Hayes, E., Foor, F., ... Schaeffer, J. M. (1999). Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells. Endocrinology, 140(8), 3790-3796.

Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells. / Mitra, Sudha Warrier; Mezey, Éva; Hunyady, B.; Chamberlain, LaShawn; Hayes, Edward; Foor, Forrest; Wang, Yining; Schonbrunn, Agnes; Schaeffer, James M.

In: Endocrinology, Vol. 140, No. 8, 1999, p. 3790-3796.

Research output: Contribution to journalArticle

Mitra, SW, Mezey, É, Hunyady, B, Chamberlain, L, Hayes, E, Foor, F, Wang, Y, Schonbrunn, A & Schaeffer, JM 1999, 'Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells', Endocrinology, vol. 140, no. 8, pp. 3790-3796.
Mitra SW, Mezey É, Hunyady B, Chamberlain L, Hayes E, Foor F et al. Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells. Endocrinology. 1999;140(8):3790-3796.
Mitra, Sudha Warrier ; Mezey, Éva ; Hunyady, B. ; Chamberlain, LaShawn ; Hayes, Edward ; Foor, Forrest ; Wang, Yining ; Schonbrunn, Agnes ; Schaeffer, James M. / Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells. In: Endocrinology. 1999 ; Vol. 140, No. 8. pp. 3790-3796.
@article{0f3d310f1ae34cc5aa7c836bef74966e,
title = "Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells",
abstract = "Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic δ-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenger RNA for both sst2 and sst5 have been reported in the rat pancreas, and the sst2A receptor protein has been localized to rat pancreatic α and pancreatic polypeptide-secreting cells in the islets as well as to pancreatic acinar cells. In this study we have used double immunostaining to show that the sst5 protein is expressed exclusively in the β-cells of rat pancreatic islets and localizes with insulin-secreting α-cells. The sst5 receptor is not colocalized with sst2A. Thus, in the rat SRIF inhibits pancreatic insulin and glucagon secretion via different sst receptor subtypes.",
author = "Mitra, {Sudha Warrier} and {\'E}va Mezey and B. Hunyady and LaShawn Chamberlain and Edward Hayes and Forrest Foor and Yining Wang and Agnes Schonbrunn and Schaeffer, {James M.}",
year = "1999",
language = "English",
volume = "140",
pages = "3790--3796",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "8",

}

TY - JOUR

T1 - Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic β-cells

AU - Mitra, Sudha Warrier

AU - Mezey, Éva

AU - Hunyady, B.

AU - Chamberlain, LaShawn

AU - Hayes, Edward

AU - Foor, Forrest

AU - Wang, Yining

AU - Schonbrunn, Agnes

AU - Schaeffer, James M.

PY - 1999

Y1 - 1999

N2 - Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic δ-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenger RNA for both sst2 and sst5 have been reported in the rat pancreas, and the sst2A receptor protein has been localized to rat pancreatic α and pancreatic polypeptide-secreting cells in the islets as well as to pancreatic acinar cells. In this study we have used double immunostaining to show that the sst5 protein is expressed exclusively in the β-cells of rat pancreatic islets and localizes with insulin-secreting α-cells. The sst5 receptor is not colocalized with sst2A. Thus, in the rat SRIF inhibits pancreatic insulin and glucagon secretion via different sst receptor subtypes.

AB - Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic δ-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenger RNA for both sst2 and sst5 have been reported in the rat pancreas, and the sst2A receptor protein has been localized to rat pancreatic α and pancreatic polypeptide-secreting cells in the islets as well as to pancreatic acinar cells. In this study we have used double immunostaining to show that the sst5 protein is expressed exclusively in the β-cells of rat pancreatic islets and localizes with insulin-secreting α-cells. The sst5 receptor is not colocalized with sst2A. Thus, in the rat SRIF inhibits pancreatic insulin and glucagon secretion via different sst receptor subtypes.

UR - http://www.scopus.com/inward/record.url?scp=0033304716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033304716&partnerID=8YFLogxK

M3 - Article

C2 - 10433240

AN - SCOPUS:0033304716

VL - 140

SP - 3790

EP - 3796

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 8

ER -