Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence

P. G. Vallet, Y. Charnay, C. Boura, J. Kiss

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence. At the light microspic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence. Immunoreactive axons were mainly found in the lateral part of the external layer. Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons. To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles. The two peptides were found colocalized in single axons. Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries. No staining was observed in tanycytes and ependymal or glial elements. Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same ~100-nm dense-core vesicles, suggesting cosecretion of these peptides.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalNeuroendocrinology
Volume53
Issue number1
Publication statusPublished - 1991

Fingerprint

Delta Sleep-Inducing Peptide
Median Eminence
Peptide Hormones
Gonadotropin-Releasing Hormone
Axons
Ependymoglial Cells
Light
Peptides
Antibodies
Secretory Vesicles
Particle Size
Neuroglia
Gold
Immunohistochemistry
Electrons
Staining and Labeling
Membranes

Keywords

  • delta sleep inducing peptide
  • electron microscopy
  • luteinizing hormone releasing hormone
  • median eminence, rat

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence. / Vallet, P. G.; Charnay, Y.; Boura, C.; Kiss, J.

In: Neuroendocrinology, Vol. 53, No. 1, 1991, p. 103-106.

Research output: Contribution to journalArticle

@article{c455bbf9963e4a23874e87c3c6d87c06,
title = "Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence",
abstract = "The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence. At the light microspic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence. Immunoreactive axons were mainly found in the lateral part of the external layer. Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons. To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles. The two peptides were found colocalized in single axons. Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries. No staining was observed in tanycytes and ependymal or glial elements. Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same ~100-nm dense-core vesicles, suggesting cosecretion of these peptides.",
keywords = "delta sleep inducing peptide, electron microscopy, luteinizing hormone releasing hormone, median eminence, rat",
author = "Vallet, {P. G.} and Y. Charnay and C. Boura and J. Kiss",
year = "1991",
language = "English",
volume = "53",
pages = "103--106",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence

AU - Vallet, P. G.

AU - Charnay, Y.

AU - Boura, C.

AU - Kiss, J.

PY - 1991

Y1 - 1991

N2 - The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence. At the light microspic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence. Immunoreactive axons were mainly found in the lateral part of the external layer. Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons. To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles. The two peptides were found colocalized in single axons. Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries. No staining was observed in tanycytes and ependymal or glial elements. Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same ~100-nm dense-core vesicles, suggesting cosecretion of these peptides.

AB - The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence. At the light microspic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence. Immunoreactive axons were mainly found in the lateral part of the external layer. Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons. To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles. The two peptides were found colocalized in single axons. Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries. No staining was observed in tanycytes and ependymal or glial elements. Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same ~100-nm dense-core vesicles, suggesting cosecretion of these peptides.

KW - delta sleep inducing peptide

KW - electron microscopy

KW - luteinizing hormone releasing hormone

KW - median eminence, rat

UR - http://www.scopus.com/inward/record.url?scp=0026079085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026079085&partnerID=8YFLogxK

M3 - Article

C2 - 2046856

AN - SCOPUS:0026079085

VL - 53

SP - 103

EP - 106

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 1

ER -