Colocalization of cocaine- And amphetamine-regulated transcript with kisspeptin and neurokinin B in the human infundibular region

Katalin Skrapits, Beáta Á Borsay, László Herczeg, Philippe Ciofi, Stephen R. Bloom, Mohammad A. Ghatei, Waljit S. Dhillo, Z. Liposits, E. Hrabovszky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Kisspeptin (KP)- and neurokinin B (NKB)- synthesizing neurons of the hypothalamic arcuate nucleus play a pivotal role in the regulation of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Unlike in rodents and sheep, the homologous KP and NKB neurons in the human infundibular region rarely express dynorphin- but often exhibit Substance P (SP) immunoreactivity, indicating remarkable species differences in the neurochemical phenotype of these neurons. In search for additional neuropeptides in human KP and NKB neurons, we carried out immunofluorescent studies on hypothalamic sections obtained from five postmenopausal women. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in 47.9±6.6% of KP-immunoreactive (IR) and 30.0±4.9% of NKB-IR perikarya and in 17.0±2.3% of KP-IR and 6.2±2.0% of NKB-IR axon varicosities. All three neuropeptides were present in 33.3±4.9% of KP-IR and 28.2±4.6% of NKB-IR somata, respectively, whereas triple-labeling showed lower incidences in KP-IR (14.3±1.8%) and NKB-IR (5.9±2.0%) axon varicosities. CART-IR KP and NKB neurons established contacts with other peptidergic cells, including GnRH-IR neurons and also sent projections to the infundibular stalk. KP and NKB fibers with CART often contained SP as well, while being distinct from CART fibers co-containing the orexigenic peptide agouti-related protein. Presence of CART in human, but not rodent, KP and NKB neurons represents a new example of species differences in the neuropeptide repertoire of mediobasal hypothalamic KP and NKB neurons. Target cells, receptor sites and physiological significance of CART in the efferent communication of KP and NKB neurons in primates require clarification.

Original languageEnglish
Article numbere103977
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 1 2014

Fingerprint

Neurokinin B
Kisspeptins
amphetamine
cocaine
Amphetamine
Cocaine
neurons
Neurons
neuropeptides
substance P
Neuropeptides
gonadotropin-releasing hormone
interspecific variation
axons
rodents
Substance P
Gonadotropin-Releasing Hormone
posterior pituitary
Axons
Agouti

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Colocalization of cocaine- And amphetamine-regulated transcript with kisspeptin and neurokinin B in the human infundibular region. / Skrapits, Katalin; Borsay, Beáta Á; Herczeg, László; Ciofi, Philippe; Bloom, Stephen R.; Ghatei, Mohammad A.; Dhillo, Waljit S.; Liposits, Z.; Hrabovszky, E.

In: PLoS One, Vol. 9, No. 8, e103977, 01.08.2014.

Research output: Contribution to journalArticle

Skrapits, Katalin ; Borsay, Beáta Á ; Herczeg, László ; Ciofi, Philippe ; Bloom, Stephen R. ; Ghatei, Mohammad A. ; Dhillo, Waljit S. ; Liposits, Z. ; Hrabovszky, E. / Colocalization of cocaine- And amphetamine-regulated transcript with kisspeptin and neurokinin B in the human infundibular region. In: PLoS One. 2014 ; Vol. 9, No. 8.
@article{984679d74f534d27ae7f91f64f283b96,
title = "Colocalization of cocaine- And amphetamine-regulated transcript with kisspeptin and neurokinin B in the human infundibular region",
abstract = "Kisspeptin (KP)- and neurokinin B (NKB)- synthesizing neurons of the hypothalamic arcuate nucleus play a pivotal role in the regulation of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Unlike in rodents and sheep, the homologous KP and NKB neurons in the human infundibular region rarely express dynorphin- but often exhibit Substance P (SP) immunoreactivity, indicating remarkable species differences in the neurochemical phenotype of these neurons. In search for additional neuropeptides in human KP and NKB neurons, we carried out immunofluorescent studies on hypothalamic sections obtained from five postmenopausal women. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in 47.9±6.6{\%} of KP-immunoreactive (IR) and 30.0±4.9{\%} of NKB-IR perikarya and in 17.0±2.3{\%} of KP-IR and 6.2±2.0{\%} of NKB-IR axon varicosities. All three neuropeptides were present in 33.3±4.9{\%} of KP-IR and 28.2±4.6{\%} of NKB-IR somata, respectively, whereas triple-labeling showed lower incidences in KP-IR (14.3±1.8{\%}) and NKB-IR (5.9±2.0{\%}) axon varicosities. CART-IR KP and NKB neurons established contacts with other peptidergic cells, including GnRH-IR neurons and also sent projections to the infundibular stalk. KP and NKB fibers with CART often contained SP as well, while being distinct from CART fibers co-containing the orexigenic peptide agouti-related protein. Presence of CART in human, but not rodent, KP and NKB neurons represents a new example of species differences in the neuropeptide repertoire of mediobasal hypothalamic KP and NKB neurons. Target cells, receptor sites and physiological significance of CART in the efferent communication of KP and NKB neurons in primates require clarification.",
author = "Katalin Skrapits and Borsay, {Be{\'a}ta {\'A}} and L{\'a}szl{\'o} Herczeg and Philippe Ciofi and Bloom, {Stephen R.} and Ghatei, {Mohammad A.} and Dhillo, {Waljit S.} and Z. Liposits and E. Hrabovszky",
year = "2014",
month = "8",
day = "1",
doi = "10.1371/journal.pone.0103977",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Colocalization of cocaine- And amphetamine-regulated transcript with kisspeptin and neurokinin B in the human infundibular region

AU - Skrapits, Katalin

AU - Borsay, Beáta Á

AU - Herczeg, László

AU - Ciofi, Philippe

AU - Bloom, Stephen R.

AU - Ghatei, Mohammad A.

AU - Dhillo, Waljit S.

AU - Liposits, Z.

AU - Hrabovszky, E.

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Kisspeptin (KP)- and neurokinin B (NKB)- synthesizing neurons of the hypothalamic arcuate nucleus play a pivotal role in the regulation of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Unlike in rodents and sheep, the homologous KP and NKB neurons in the human infundibular region rarely express dynorphin- but often exhibit Substance P (SP) immunoreactivity, indicating remarkable species differences in the neurochemical phenotype of these neurons. In search for additional neuropeptides in human KP and NKB neurons, we carried out immunofluorescent studies on hypothalamic sections obtained from five postmenopausal women. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in 47.9±6.6% of KP-immunoreactive (IR) and 30.0±4.9% of NKB-IR perikarya and in 17.0±2.3% of KP-IR and 6.2±2.0% of NKB-IR axon varicosities. All three neuropeptides were present in 33.3±4.9% of KP-IR and 28.2±4.6% of NKB-IR somata, respectively, whereas triple-labeling showed lower incidences in KP-IR (14.3±1.8%) and NKB-IR (5.9±2.0%) axon varicosities. CART-IR KP and NKB neurons established contacts with other peptidergic cells, including GnRH-IR neurons and also sent projections to the infundibular stalk. KP and NKB fibers with CART often contained SP as well, while being distinct from CART fibers co-containing the orexigenic peptide agouti-related protein. Presence of CART in human, but not rodent, KP and NKB neurons represents a new example of species differences in the neuropeptide repertoire of mediobasal hypothalamic KP and NKB neurons. Target cells, receptor sites and physiological significance of CART in the efferent communication of KP and NKB neurons in primates require clarification.

AB - Kisspeptin (KP)- and neurokinin B (NKB)- synthesizing neurons of the hypothalamic arcuate nucleus play a pivotal role in the regulation of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Unlike in rodents and sheep, the homologous KP and NKB neurons in the human infundibular region rarely express dynorphin- but often exhibit Substance P (SP) immunoreactivity, indicating remarkable species differences in the neurochemical phenotype of these neurons. In search for additional neuropeptides in human KP and NKB neurons, we carried out immunofluorescent studies on hypothalamic sections obtained from five postmenopausal women. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in 47.9±6.6% of KP-immunoreactive (IR) and 30.0±4.9% of NKB-IR perikarya and in 17.0±2.3% of KP-IR and 6.2±2.0% of NKB-IR axon varicosities. All three neuropeptides were present in 33.3±4.9% of KP-IR and 28.2±4.6% of NKB-IR somata, respectively, whereas triple-labeling showed lower incidences in KP-IR (14.3±1.8%) and NKB-IR (5.9±2.0%) axon varicosities. CART-IR KP and NKB neurons established contacts with other peptidergic cells, including GnRH-IR neurons and also sent projections to the infundibular stalk. KP and NKB fibers with CART often contained SP as well, while being distinct from CART fibers co-containing the orexigenic peptide agouti-related protein. Presence of CART in human, but not rodent, KP and NKB neurons represents a new example of species differences in the neuropeptide repertoire of mediobasal hypothalamic KP and NKB neurons. Target cells, receptor sites and physiological significance of CART in the efferent communication of KP and NKB neurons in primates require clarification.

UR - http://www.scopus.com/inward/record.url?scp=84905483124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905483124&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0103977

DO - 10.1371/journal.pone.0103977

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e103977

ER -