Colloidal gold-labeled lipoprotein binding and internalization in adrenocortical cells in vitro

Ida E. Tóth, Dezsõ Szabó, Katalin Sz Szalay, Angéla Gyéval, Lajos G. Szollár, Edit Gláz

Research output: Contribution to journalArticle

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Abstract

Human (normal and adenomatous) and rat (normal) adrenocortical cells were incubated in vitro with colloidal gold labeled low-density (LDL-Au) and high-density (HDL-Au) lipoproteins, respectively, in order to visualize lipoprotein binding and internalization at an electron microscopic level. Both normal and adenomatous human adrenocortical cells accumulated LDL-Au by receptor-mediated endocytosis via coated pits, coated vesicles, noncoated vesicles, and lysosomes. HDL-Au was not internalized. In rat adrenocortical cells, both HDL-Au and to a lesser extent LDL-Au were internalized. It is concluded that LDL-Au and HDL-Au conjugates can be used to identify lipoprotein receptors and to follow lipoprotein internalization in adrenocortical cells.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalClinical Biochemistry
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 1988

Keywords

  • HDL-metabolism
  • LDL-metabolism
  • adrenal adenomas
  • electron microscopy
  • human and rat adrenocortical cells

ASJC Scopus subject areas

  • Clinical Biochemistry

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