Collective motion of cells mediates segregation and pattern formation in co-cultures

Elod Méhes, Enys Mones, Valéria Németh, T. Vicsek

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Pattern formation by segregation of cell types is an important process during embryonic development. We show that an experimentally yet unexplored mechanism based on collective motility of segregating cells enhances the effects of known pattern formation mechanisms such as differential adhesion, mechanochemical interactions or cell migration directed by morphogens. To study in vitro cell segregation we use time-lapse videomicroscopy and quantitative analysis of the main features of the motion of individual cells or groups. Our observations have been extensive, typically involving the investigation of the development of patterns containing up to 200,000 cells. By either comparing keratocyte types with different collective motility characteristics or increasing cells' directional persistence by the inhibition of Rac1 GTP-ase we demonstrate that enhanced collective cell motility results in faster cell segregation leading to the formation of more extensive patterns. The growth of the characteristic scale of patterns generally follows an algebraic scaling law with exponent values up to 0.74 in the presence of collective motion, compared to significantly smaller exponents in case of diffusive motion.

Original languageEnglish
Article numbere31711
JournalPLoS One
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 16 2012

Fingerprint

Cell Separation
Scaling laws
coculture
Coculture Techniques
Guanosine Triphosphate
Cell Movement
Adhesion
Chemical analysis
cell movement
Video Microscopy
cells
Embryonic Development
guanosinetriphosphatase
Growth
in vitro studies
adhesion
quantitative analysis
embryogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Collective motion of cells mediates segregation and pattern formation in co-cultures. / Méhes, Elod; Mones, Enys; Németh, Valéria; Vicsek, T.

In: PLoS One, Vol. 7, No. 2, e31711, 16.02.2012.

Research output: Contribution to journalArticle

Méhes, Elod ; Mones, Enys ; Németh, Valéria ; Vicsek, T. / Collective motion of cells mediates segregation and pattern formation in co-cultures. In: PLoS One. 2012 ; Vol. 7, No. 2.
@article{5b7cd7c4e8a0467fb6c7f9315e3f5bea,
title = "Collective motion of cells mediates segregation and pattern formation in co-cultures",
abstract = "Pattern formation by segregation of cell types is an important process during embryonic development. We show that an experimentally yet unexplored mechanism based on collective motility of segregating cells enhances the effects of known pattern formation mechanisms such as differential adhesion, mechanochemical interactions or cell migration directed by morphogens. To study in vitro cell segregation we use time-lapse videomicroscopy and quantitative analysis of the main features of the motion of individual cells or groups. Our observations have been extensive, typically involving the investigation of the development of patterns containing up to 200,000 cells. By either comparing keratocyte types with different collective motility characteristics or increasing cells' directional persistence by the inhibition of Rac1 GTP-ase we demonstrate that enhanced collective cell motility results in faster cell segregation leading to the formation of more extensive patterns. The growth of the characteristic scale of patterns generally follows an algebraic scaling law with exponent values up to 0.74 in the presence of collective motion, compared to significantly smaller exponents in case of diffusive motion.",
author = "Elod M{\'e}hes and Enys Mones and Val{\'e}ria N{\'e}meth and T. Vicsek",
year = "2012",
month = "2",
day = "16",
doi = "10.1371/journal.pone.0031711",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Collective motion of cells mediates segregation and pattern formation in co-cultures

AU - Méhes, Elod

AU - Mones, Enys

AU - Németh, Valéria

AU - Vicsek, T.

PY - 2012/2/16

Y1 - 2012/2/16

N2 - Pattern formation by segregation of cell types is an important process during embryonic development. We show that an experimentally yet unexplored mechanism based on collective motility of segregating cells enhances the effects of known pattern formation mechanisms such as differential adhesion, mechanochemical interactions or cell migration directed by morphogens. To study in vitro cell segregation we use time-lapse videomicroscopy and quantitative analysis of the main features of the motion of individual cells or groups. Our observations have been extensive, typically involving the investigation of the development of patterns containing up to 200,000 cells. By either comparing keratocyte types with different collective motility characteristics or increasing cells' directional persistence by the inhibition of Rac1 GTP-ase we demonstrate that enhanced collective cell motility results in faster cell segregation leading to the formation of more extensive patterns. The growth of the characteristic scale of patterns generally follows an algebraic scaling law with exponent values up to 0.74 in the presence of collective motion, compared to significantly smaller exponents in case of diffusive motion.

AB - Pattern formation by segregation of cell types is an important process during embryonic development. We show that an experimentally yet unexplored mechanism based on collective motility of segregating cells enhances the effects of known pattern formation mechanisms such as differential adhesion, mechanochemical interactions or cell migration directed by morphogens. To study in vitro cell segregation we use time-lapse videomicroscopy and quantitative analysis of the main features of the motion of individual cells or groups. Our observations have been extensive, typically involving the investigation of the development of patterns containing up to 200,000 cells. By either comparing keratocyte types with different collective motility characteristics or increasing cells' directional persistence by the inhibition of Rac1 GTP-ase we demonstrate that enhanced collective cell motility results in faster cell segregation leading to the formation of more extensive patterns. The growth of the characteristic scale of patterns generally follows an algebraic scaling law with exponent values up to 0.74 in the presence of collective motion, compared to significantly smaller exponents in case of diffusive motion.

UR - http://www.scopus.com/inward/record.url?scp=84857135404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857135404&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0031711

DO - 10.1371/journal.pone.0031711

M3 - Article

C2 - 22359617

AN - SCOPUS:84857135404

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e31711

ER -