Colistin resistance associated with outer membrane protein change in Klebsiella pneumoniae and Enterobacter asburiae

Béla Kádár, Béla Kocsis, A. Tóth, Katalin Kristof, Péter Felso, Béla Kocsis, D. Szabó

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by twodimensional gel electrophoresis and in-gel digestion. The MALDI-TOF/MS analysis of OMPs in the colistin-susceptible K. pneumoniae found 16 kDa proteins belonging to the LysM domain/BON superfamily, as well as DNA starvation proteins, whereas OmpX and OmpW were detected in the colistin-resistant counterpart strains. OmpC and OmpW were detected in the colistin-susceptible E. asburiae, whereas OmpA and OmpX were identified in the colistin-resistant counterpart. This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae.

Original languageEnglish
Pages (from-to)217-227
Number of pages11
JournalActa Microbiologica et Immunologica Hungarica
Volume64
Issue number2
DOIs
Publication statusPublished - Jun 1 2017

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Colistin
Enterobacter
Klebsiella pneumoniae
Membrane Proteins
Mass Spectrometry
Lasers
Gels
Enterobacteriaceae
Starvation
Cell Wall
Electrophoresis
Digestion
Proteins

Keywords

  • Colistin resistance
  • MALDI-TOF/MS
  • Outer membrane protein

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Colistin resistance associated with outer membrane protein change in Klebsiella pneumoniae and Enterobacter asburiae. / Kádár, Béla; Kocsis, Béla; Tóth, A.; Kristof, Katalin; Felso, Péter; Kocsis, Béla; Szabó, D.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 64, No. 2, 01.06.2017, p. 217-227.

Research output: Contribution to journalArticle

Kádár, Béla ; Kocsis, Béla ; Tóth, A. ; Kristof, Katalin ; Felso, Péter ; Kocsis, Béla ; Szabó, D. / Colistin resistance associated with outer membrane protein change in Klebsiella pneumoniae and Enterobacter asburiae. In: Acta Microbiologica et Immunologica Hungarica. 2017 ; Vol. 64, No. 2. pp. 217-227.
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