Cold storage of shoot cultures and mycorrhizal inoculation of plantlets

Ildikó Balla, Endre Szucs, Ibolya Vörös, Borbála Bíró

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two methods have been examined to reduce labour requirement or to extend the acclimatization period: (1) To prepare large quantity of in vitro cultures for rooting using a cold storage period before the rooting phase. (2) To shorten the growth time and to improve the vigour of micropropagated rootstocks by mycorrhizal inoculation. Both methods can be used to reduce the labour requirement in the spring time, and to extend the transplantation period of the acclimatized plants to the outdoors till the beginning of July. The in vitro cultures can be stored at 4°C before rooting. The cold treatment should begin at half time of the multiplication period or the elongation period. In this case there is no harmful influence on rooting. Significant growth stimulating effect can be achieved using selected clones of mycorrhiza lines for inoculation of the plantlets.

Original languageEnglish
Title of host publicationI International Symposium on Acclimatization and Establishment of Micropropagted Plants
PublisherInternational Society for Horticultural Science
Pages185-188
Number of pages4
ISBN (Print)9789066050082
DOIs
Publication statusPublished - Jan 1 2003

Publication series

NameActa Horticulturae
Volume616
ISSN (Print)0567-7572

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Keywords

  • Acclimatization period
  • Forest-trees
  • Fruit-trees
  • Micropropagation
  • Mycorrhizae
  • Rootstocks

ASJC Scopus subject areas

  • Horticulture

Cite this

Balla, I., Szucs, E., Vörös, I., & Bíró, B. (2003). Cold storage of shoot cultures and mycorrhizal inoculation of plantlets. In I International Symposium on Acclimatization and Establishment of Micropropagted Plants (pp. 185-188). (Acta Horticulturae; Vol. 616). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2003.616.21