Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels

Studies on callus cultures

Ildikó Vashegyi, Zsuzsa Marozsán-Tóth, G. Galiba, Petre I. Dobrev, Radomira Vankova, Balázs Tóth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.

Original languageEnglish
Pages (from-to)337-349
Number of pages13
JournalMolecular Biotechnology
Volume54
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Dicamba
Indoleacetic Acids
Hormones
Bony Callus
Hordeum
Gene expression
Freezing
Chemical activation
Gene Expression
Tissue culture
Abscisic Acid
Acids
Salicylic Acid
Chemical analysis
Salicylic acid
Hardening
Genes
Acclimatization
Cold-Shock Response
Light

Keywords

  • ABA
  • Barley
  • Callus
  • CBF
  • Cold
  • COR14b
  • Dicamba
  • IAA

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels : Studies on callus cultures. / Vashegyi, Ildikó; Marozsán-Tóth, Zsuzsa; Galiba, G.; Dobrev, Petre I.; Vankova, Radomira; Tóth, Balázs.

In: Molecular Biotechnology, Vol. 54, No. 2, 06.2013, p. 337-349.

Research output: Contribution to journalArticle

Vashegyi, Ildikó ; Marozsán-Tóth, Zsuzsa ; Galiba, G. ; Dobrev, Petre I. ; Vankova, Radomira ; Tóth, Balázs. / Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels : Studies on callus cultures. In: Molecular Biotechnology. 2013 ; Vol. 54, No. 2. pp. 337-349.
@article{ae902b091af5401db63311bfac739579,
title = "Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels: Studies on callus cultures",
abstract = "In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.",
keywords = "ABA, Barley, Callus, CBF, Cold, COR14b, Dicamba, IAA",
author = "Ildik{\'o} Vashegyi and Zsuzsa Marozs{\'a}n-T{\'o}th and G. Galiba and Dobrev, {Petre I.} and Radomira Vankova and Bal{\'a}zs T{\'o}th",
year = "2013",
month = "6",
doi = "10.1007/s12033-012-9569-9",
language = "English",
volume = "54",
pages = "337--349",
journal = "Molecular Biotechnology",
issn = "1073-6085",
publisher = "Humana Press",
number = "2",

}

TY - JOUR

T1 - Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels

T2 - Studies on callus cultures

AU - Vashegyi, Ildikó

AU - Marozsán-Tóth, Zsuzsa

AU - Galiba, G.

AU - Dobrev, Petre I.

AU - Vankova, Radomira

AU - Tóth, Balázs

PY - 2013/6

Y1 - 2013/6

N2 - In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.

AB - In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.

KW - ABA

KW - Barley

KW - Callus

KW - CBF

KW - Cold

KW - COR14b

KW - Dicamba

KW - IAA

UR - http://www.scopus.com/inward/record.url?scp=84882859615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882859615&partnerID=8YFLogxK

U2 - 10.1007/s12033-012-9569-9

DO - 10.1007/s12033-012-9569-9

M3 - Article

VL - 54

SP - 337

EP - 349

JO - Molecular Biotechnology

JF - Molecular Biotechnology

SN - 1073-6085

IS - 2

ER -