Colchicine induced embryogenesis in maize

Bohuš Obert, B. Barnabás

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The present study involves in vitro androgenesis of Zea mays L. using anther culture. We tested combinations of single factors and their influence on microspore induction. Embryogenic induction of microspores within anthers in in vitro conditions was the best when combination of cold treatment, TIBA (0.1 mg l-1) in media and colchicine (0.02% during first 3 days of culture) was applied, but colchicine alone can be factor, which can stimulate or initiate embryogenesis in anther culture of maize.

Original languageEnglish
Pages (from-to)283-285
Number of pages3
JournalPlant Cell, Tissue and Organ Culture
Volume77
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

2,3,5-triiodobenzoic acid
anther culture
colchicine
Colchicine
microspores
Zea mays
Embryonic Development
embryogenesis
androgenesis
corn
in vitro studies
anthers
In Vitro Techniques
cold treatment

Keywords

  • Anther culture
  • Colchicine
  • Embryogenesis
  • Microspore
  • Zea mays L.

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

Colchicine induced embryogenesis in maize. / Obert, Bohuš; Barnabás, B.

In: Plant Cell, Tissue and Organ Culture, Vol. 77, No. 3, 06.2004, p. 283-285.

Research output: Contribution to journalArticle

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