The present study involves in vitro androgenesis of Zea mays L. using anther culture. We tested combinations of single factors and their influence on microspore induction. Embryogenic induction of microspores within anthers in in vitro conditions was the best when combination of cold treatment, TIBA (0.1 mg l -1 ) in media and colchicine (0.02% during first 3 days of culture) was applied, but colchicine alone can be factor, which can stimulate or initiate embryogenesis in anther culture of maize.
|Number of pages||3|
|Journal||Plant Cell, Tissue and Organ Culture|
|Publication status||Published - Jun 2004|
- Anther culture
- Zea mays L.
ASJC Scopus subject areas