Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants

Magdalena Dory, Elizabeth Hatzimasoura, Brigitta M. Kállai, Szilvia K. Nagy, Katalin Jäger, Zsuzsanna Darula, Tímea V. Nádai, Tamás Mészáros, Enrique López-Juez, Beáta Barnabás, Klaus Palme, László Bögre, Franck A. Ditengou, Róbert Dóczi

Research output: Contribution to journalLetter

13 Citations (Scopus)

Abstract

Plant growth flexibly adapts to environmental conditions, implying cross-talk between environmental signalling and developmental regulation. Here, we show that the PIN auxin efflux carrier family possesses three highly conserved putative mitogen-activated protein kinase (MAPK) sites adjacent to the phosphorylation sites of the well-characterised AGC kinase PINOID, which regulates the polar localisation of PINs and directional auxin transport, thereby underpinning organ growth. The conserved sites of PIN1 are phosphorylated in vitro by two environmentally activated MAPKs, MPK4 and MPK6. In contrast to AGC kinases, MAPK-mediated phosphorylation of PIN1 at adjacent sites leads to a partial loss of the plasma membrane localisation of PIN1. MAPK-mediated modulation of PIN trafficking may participate in environmental adjustment of plant growth.

Original languageEnglish
Pages (from-to)89-102
Number of pages14
JournalFEBS letters
Volume592
Issue number1
DOIs
Publication statusPublished - Jan 2018

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Keywords

  • Arabidopsis
  • MAP kinase
  • PIN
  • protein phosphorylation
  • signalling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dory, M., Hatzimasoura, E., Kállai, B. M., Nagy, S. K., Jäger, K., Darula, Z., Nádai, T. V., Mészáros, T., López-Juez, E., Barnabás, B., Palme, K., Bögre, L., Ditengou, F. A., & Dóczi, R. (2018). Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants. FEBS letters, 592(1), 89-102. https://doi.org/10.1002/1873-3468.12929