Coenzyme production using immobilized enzymes. II. Factors affecting the continuous production of NADP

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Abstract

The effects of certain factors on the continuous production of NADP+ with immobilized NAD+ kinase were studied. In a column reactor, the maximum NADP+ productivity was experienced at pH 8.0. The presence of 10-12 mM Mg2+ ion appeared to be optimum for maximum productivity. K+ ion at 1-5 mM behaved as an activator. Mn2+ and Zn2+ ions proved effective activators of the immobilized NAD+ kinase. Systems of up to three or four columns were most effective in NADP+ production.

Original languageEnglish
Pages (from-to)236-239
Number of pages4
JournalEnzyme and Microbial Technology
Volume16
Issue number3
DOIs
Publication statusPublished - 1994

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Coenzymes
Immobilized Enzymes
NADP
Enzymes
Ions
Productivity
NAD kinase

Keywords

  • NAD kinase, immobilized
  • NADP production, factors affecting

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Coenzyme production using immobilized enzymes. II. Factors affecting the continuous production of NADP",
abstract = "The effects of certain factors on the continuous production of NADP+ with immobilized NAD+ kinase were studied. In a column reactor, the maximum NADP+ productivity was experienced at pH 8.0. The presence of 10-12 mM Mg2+ ion appeared to be optimum for maximum productivity. K+ ion at 1-5 mM behaved as an activator. Mn2+ and Zn2+ ions proved effective activators of the immobilized NAD+ kinase. Systems of up to three or four columns were most effective in NADP+ production.",
keywords = "NAD kinase, immobilized, NADP production, factors affecting",
author = "Simon, {L. M.} and M. Kotorm{\'a}n and B. Szaj{\'a}ni",
year = "1994",
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journal = "Enzyme and Microbial Technology",
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AU - Kotormán, M.

AU - Szajáni, B.

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AB - The effects of certain factors on the continuous production of NADP+ with immobilized NAD+ kinase were studied. In a column reactor, the maximum NADP+ productivity was experienced at pH 8.0. The presence of 10-12 mM Mg2+ ion appeared to be optimum for maximum productivity. K+ ion at 1-5 mM behaved as an activator. Mn2+ and Zn2+ ions proved effective activators of the immobilized NAD+ kinase. Systems of up to three or four columns were most effective in NADP+ production.

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KW - NADP production, factors affecting

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