Coenzyme A is required for rat liver fatty acid synthetase activity.

T. C. Linn, M. J. Stark, P. Srere

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Inhibition of highly purified rat liver fatty acid synthetase occurs when it is assayed in the presence of the ATP citrate lyase reaction components. Citrate, Mg2+, ATP, and ATP citrate lyase were all necessary for the inhibition to take place. Inhibition was prevented by hydroxycitrate, a competitive inhibitor for ATP citrate lyase. The length of time for the onset of inhibition to take place was proportional to the ratio of ATP citrate lyase activity to the fatty acid synthetase activity. The inhibition was reversed by the addition of coenzyme A. This indicates a reaction mechanism for fatty acid synthetase which involves free coenzyme A. Two possible roles for CoA are discussed, one as an allosteric activator and the other in the cleavage of palmitoyl enzyme in the last step of the reaction.

Original languageEnglish
Pages (from-to)1388-1392
Number of pages5
JournalJournal of Biological Chemistry
Volume255
Issue number4
Publication statusPublished - Feb 25 1980

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ATP Citrate (pro-S)-Lyase
Fatty Acid Synthases
Coenzyme A
Liver
Rats
Citric Acid
Adenosine Triphosphate
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Coenzyme A is required for rat liver fatty acid synthetase activity. / Linn, T. C.; Stark, M. J.; Srere, P.

In: Journal of Biological Chemistry, Vol. 255, No. 4, 25.02.1980, p. 1388-1392.

Research output: Contribution to journalArticle

Linn, T. C. ; Stark, M. J. ; Srere, P. / Coenzyme A is required for rat liver fatty acid synthetase activity. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 4. pp. 1388-1392.
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